Abstract:
Bunium persicum belongs to the Apiaceae family. This plant throughout the world is called by different names such as “Black caraway”, ''Black zire''“Shah zira”. The current study was designed to assess the biological activities of Bunium persicum seed extracts. To evaluate the antioxidant, antibacterial, and hemolytic activity distilled water, ethanol, 70 % hydro ethanol, ethyl acetate, 40% HCl, and n-Hexane extracts of Bunium persicum seed were used. The extract's antioxidant activity was checked by DP PH radical scavenging activity. Total phenolic content wasanalyzed by FolinCiocalteu method and total flavonoid content were analyzed by Aluminum chloride colorimetric assay. Antibacterial study was carried out by agar well diffusion method and biofilm inhibition by microtiter plate method. The cytotoxicity study was assessed by hemolytic assay. In DPPH assay the order of free radical scavenging activity of extracts was hydro ethanol > ethanol> distilled water > HCl > ethyl acetate >n-Hexane. The hydro ethanol extract exhibited highesttotal phenolic content (401.39 ± 1.07g gallic acid equivalent/ g dry weight) and total flavonoid content (207.35 ± 1.79 catechin equivalent /g dry weight) as compared to other extracts. The antibacterial activity against gram-negative bacteria E. coli and gram-positive bacteria S. aureus was checked. Against E. coli distilled water extract showed (16.11 ± 0.11), hydroethanol, ethanol, and HCl showed (10.16 ± 0.15) mm zone of inhibition. Against S. aureus ethanol and hydro ethanol extract(20.10 ± 0.10), distilled water (13.22± 0.21), and HCl extract showed (10.16± 0.15) mm zone of inhibition whereas n-Hexane and ethyl acetate extracts exhibited no antibacterial activity. All extracts of Bunium persicum seed showed biofilm inhibition activity against E. coli and S. aureus. In hemolytic assay HCl and n-Hexane extracts showed slight cytotoxic activity whereas other extracts were non-toxic. Results were analyzed statistically using one way analysis of variance (ANOVA) and mean comparison was checked by applying t-Test. Results indicated that hydro ethanol extract of Bunium persicum seed exhibited highest DPPH scavenging activity and have highest TPC and TFC. It also showed antibacterial and biofilm inhibition activity and non-toxic in nature hence can be used for therapeutic purposes.
Page(s):
277-277
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
antioxidant
,
cytotoxicity
,
antibacterial
,
biofilm
,
Bunium persicum