Author(s):
1. Maryam Khalid:
Department of Biochemistry, IBBB, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
2. Gull-e-Faran:
Department of Biochemistry, IBBB, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
Abstract:
Fibrinolytic enzymes are responsible for cleavage of fibrin mesh in thrombus clots, which are the primary causative agents in cardiovascular diseases. Obstruction of blood vessels with clots causes acute myocardial infarction and ischemic stroke which are the leading causes of death. Thrombolytic agents are administered to liquefy the blood lump. Production of alkaline protease, a thrombolytic agent and fibrinolytic enzyme from Bacillus subtilis was carried out in this research work. Bacterial samples were inoculated on Nutrient agar media plates and fermentation media was used for extraction of Bacillus subtilis. Rape seed meal was used as a substrate for the bacterial strain. Bacterial strain was used for the production, propagation and purification of alkaline protease. The enzyme alkaline protease was purified by ion exchange column chromatography. The protein was characterized using SDS-PAGE. The enzyme produced by the bacterial strain resulted in enzyme activity of 301 U/ml with 57.88 U/mg specific activity, 5.2 mg/ml protein, 3.18-fold purification and 82.01% recovery while the molecular weight of the enzyme was approximately 27kDa. Alkaline protease produced from the bacterial strain give its best performance at pH 7.0. Thermal stability between 30ºC to 50ºC was shown by the alkaline protease. For this purpose, growth of Bacillus subtilis was optimized at various parameters (pH, temperature, substrate concentration and fermentation time). By using these optimized conditions maximum growth of Bacillus subtilis would be obtained and good quality alkaline protease with cheap cost can be produced. It was concluded that it may be potentially useful for industrial purposes.
Page(s):
89-89
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023
Keywords:
Bacillus subtilis
,
alkaline protease
,
Thrombolytic agent
,
Rape seed meal
,
Proteolytic enzyme
References:
References are not available for this document.
Citations
Citations are not available for this document.