Abiotic factors affecting in vitro pollen germination in sainfoin (Onobrychis viciifolia Scop.). | [Pakistan Journal of Botany • 2003]

Author(s):

1. Cengiz Sancak: Department of Field Crops, Faculty of Agriculture, University of Ankara, Diskapi, Ankara, Turkey

2. Sati Cocu: Department of Field Crops, Faculty of Agriculture, University of Ankara, Diskapi, Ankara, Turkey

3. Kahlid Mahmood Khawar: Department of Field Crops, Faculty of Agriculture, University of Ankara, Diskapi, Ankara, Turkey

4. Sebahattin Ozcan: Department of Field Crops, Faculty of Agriculture, University of Ankara, Diskapi, Ankara, Turkey

Abstract:

A reliable in vitro method for germination of sainfoin pollen grains has been developed. Highest pollen germination rate of 96% with mean pollen tube length of 223.5 mm was obtained on medium containing of 100 g/I sucrose, 200 mg/I KNO3, 150 mg/I H3BO3, 150 mg/I MgSO4, 150 mg/I Ca(NO3)2, 175 mg/I PEG 4000 and stigma extract at pH 6.5.

Page(s): 463-471

DOI: DOI not available

Published: Journal: Pakistan Journal of Botany, Volume: 35, Issue: 4, Year: 2003

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