Abstract:
Objective: To compare superoxide dismutase 1 (SOD1) levels in diabetes mellitus patients with and without neuropathy. Study Design: Cross-sectional comparative study. Place and Duration of Study: Multidisciplinary Lab-1 of the Department of Biochemistry and Molecular Biology, Army Medical College, in cooperation with Pak-Emirates Military Hospital, Rawalpindi, Pakistan, from January 2020 to January 2021. Methodology: Eighty-four subjects were enrolled through non-probability purposive sampling technique. They were further divided into three groups. Patients with diabetic neuropathy were labelled as the group ?, and patients with diabetes mellitus without neuropathy were included in group ??. While group ??? was comprised of healthy individuals and taken as control. Biochemical parameters included fasting blood glucose levels and HbA1c. Superoxide dismutase-1 levels were measured by enzyme-linked immunosorbent assay (ELISA). Data were evaluated by SPSS version 22.0 and presented in percentage and mean ± standard deviation (SD). Independent sample t-test and one way ANOVA followed by the post-hoc Tukey test were used for group comparison. Results: Mean level of SOD1 was signi cantly higher in group ? as compared to group ?? and ??? (p<0.001). Statistically signi cant di erence was observed among groups ? and ?? (p0=.002), ? and ??? (p<0.00),1and ?? and ??? (0p.=017). Mean levels of SOD1 were also signi cantly increased with poor glycemic control (p<0.001). Conclusion: SOD1 level was considerably high in patients with diabetes mellitus with neuropathy in comparison to diabetics without neuropathy and healthy individuals.
Keywords:
Diabetes mellitus
,
ELISA
,
Diabetes mellitus
,
Diabetic neuropathy
,
Superoxide dismutase 1SOD1