Transcriptome Profiling of Potassium Starvation Responsiveness in Flax (Linum Usitatissimum L.). | [Pakistan Journal of Botany • 2019]

Author(s):

1. WENGONG HUANG: College of Life Science, Northeast Forestry University, Harbin 150040, China: Institute of Industrial Crops, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China

2. SHUQUAN ZHANG: Institute of Industrial Crops, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China

3. Guangwen Wu: Institute of Industrial Crops, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China

4. YING YU: Institute of Industrial Crops, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China

5. Chuanying Ren: Food Processing Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China

6. QINGHUA KANG: Institute of Industrial Crops, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China

7. YAN LIU: Institute of Industrial Crops, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China

8. CHUNBO LIANG: Institute of Industrial Crops, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China

9. LIGUO ZHANG: Institute of Industrial Crops, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China

10. YAGUANG ZHAN: College of Life Science, Northeast Forestry University, Harbin 150040, China

Abstract:

Potassium (K) is an essential plant nutrient. The significance of potassium can be appreciated by observing plants grown under K+-deficient conditions, which greatly restricts growth and development and results in loss of crop quality and yield. Flax (Linum usttatissimum L.) is a significant economic crop that is often negatively impacted by K+ deficiency. To highlight K+ deficiency response mechanisms and increase flax potassium absorption and utilization ratio, flax variety Sofie was studied by studying seedlings after growing with or without K+ supply for 12h and 96h. cDNA was sequenced using an Illumina system. Genes involved in different regulatory mechanisms of K+-uptake during 12h and 96h stress were identified. In the K+-starvation group, 1154 and 247 differentially expressed genes (DEGs) were discovered after 12h and 96h of starvation, respectively. The results showed that 546 DEGs were annotated to 46 transcription factor families, 262 DEGs were annotated to signal transduction proteins or as participants in signal transduction pathways, 102 DEGs were annotated to hormone response proteins and 106 DEGs were annotated to transporter proteins. Multiple ion channels were also identified among the DEGs, including ion channel proteins homologous to AKT channels, KAT channels and CNG ion channels. This is the first study to analyze molecular response mechanisms of the flax transcriptome in response to K+ deficiency. These data provide numerous candidate genes with K+ deficiency that should guide future studies to elucidate plant strategies for adaptation to potassium deficiency.

Page(s): 865-878

DOI: 10.30848/PJB2019-3(28)

Published: Journal: Pakistan Journal of Botany, Volume: 51, Issue: 3, Year: 2019

Keywords:

RNASeq , differentially expressed genes , Flax , Potassium deficiency

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