An economical and efficient method for mass propagation of Ixora coccinea. | [Pakistan Journal of Botany • 2004]

Author(s):

1. Saif Ullah Khan: Plant Tissue Culture and Biotechnology Division, International Centre for Chemical Sciences, HEJ Research Institute of Chemistry, University of Karachi, Karachi, Pakistan

2. Mariam Iftikhar: Plant Tissue Culture and Biotechnology Division, International Centre for Chemical Sciences, HEJ Research Institute of Chemistry, University of Karachi, Karachi, Pakistan

3. Bushra Saeed: Plant Tissue Culture and Biotechnology Division, International Centre for Chemical Sciences, HEJ Research Institute of Chemistry, University of Karachi, Karachi, Pakistan

Abstract:

A simple and efficient micropropagation protocol was developed for Ixora coccinea cv. Super King, a beautiful ornamental plant using shoot tip and nodal segments as explants. The explants were cultured in Woody Plant Medium (WPM) supplemented with 0.5 mg/L BAP incubated for 10 weeks initially and subcultured onto fresh medium after every 4 weeks. The proliferating clusters were cultured in WPM basal medium fortified with Peptone (40 mg/I) and 3% sucrose for elongation of shoots, formation of root initials and further proliferation of axillary shoots. Separation and culturing of elongated shoots in fresh medium for 2 to 3 weeks yielded 7 to 8 cm long plantlets which were acclimatized in polytunnels. The most suitable medium for rooting was half-WPM enriched with IBA at a concentration of 0.05 mg/L. An increase in axillary shoot number of up to 10 per shoot were observed. This exponential rise in plants was very encouraging for the development of an efficient commercial micropropagation system.

Page(s): 751-756

DOI: DOI not available

Published: Journal: Pakistan Journal of Botany, Volume: 36, Issue: 4, Year: 2004

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