Agrobacterium mediated transformation of soybean (Glycine max L.): Some conditions standardization. | [Pakistan Journal of Botany • 2010]

Author(s):

1. Muhammad Zia: Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan 45320

2. Zarrin Fatima Rizvi: Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan 45320

3. Riaz-Ur-Rehman: Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan 45320

4. M. Fayyaz Chaudhary: Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan 45320

Abstract:

Present study was aimed to standardize some transformation conditions for soybean cultivar NARC-4 using Agrobacterium tumefaciens strain EHA 101 harboring pGUSintNPTII. Soybean half seed cotyledonary node method was opted. It was found that explant preparation in Agrosuspension culture resulted in highest transformation efficiency (48.3%) than in infection medium and water. One hour infection time was found optimum (55.9% transformation efficiency) in culture OD600 1.0. Co-cultivation of soybean half seed explants with Agrobacterium for five days showed better results as compared with three and four days. Two hr washing in washing medium containing 1g/L cefotaxime controlled prevalence of Agrobacterium in further steps. It was found that varying kanamycin concentration in selection medium resulted in high survival rate of transformed shoots. NARC-4 soybean cultivar showed better transformation efficiency than NARC-7 when genotype dependency was examined using Agrobacterium strain EHA101.

Page(s): 2269-2279

DOI: DOI not available

Published: Journal: Pakistan Journal of Botany, Volume: 42, Issue: 4, Year: 2010

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