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Transcriptomic Comparative Analysis of Two Breeds of Mongolian Sheep at 16-day Embryos
Author(s):
1. Hong Su: College of Veterinary Medicine, Inner Mongolia Agricultural University, China; Animal Embryo and Developmental Engineering Key Laboratory of Higher Education Institutions of Inner Mongolia Autonomous Region, China
2. Lu Chen: College of Veterinary Medicine, Inner Mongolia Agricultural University, China; Inner Mongolia Autonomous Region Key Laboratory of Basic Veterinary Medicine, China China
3. Wenrui Guo: College of Veterinary Medicine, Inner Mongolia Agricultural University, China
4. Daqing Wang: College of Veterinary Medicine, Inner Mongolia Agricultural University, China; Inner Mongolia Autonomous Region Key Laboratory of Basic Veterinary Medicine, China China
5. Aolei Dou: College of Veterinary Medicine, Inner Mongolia Agricultural University, China; Animal Embryo and Developmental Engineering Key Laboratory of Higher Education Institutions of Inner Mongolia Autonomous Region, China
6. Jie Su: College of Veterinary Medicine, Inner Mongolia Agricultural University, China
7. Yanyan Yang: Institute of Animal Husbandry, Inner Mongolia Academy of Agriculture and Animal Husbandry
8. Ying Tian: Institute of Animal Husbandry, Inner Mongolia Academy of Agriculture and Animal Husbandry
9. Tingyi He: Institute of Animal Husbandry, Inner Mongolia Academy of Agriculture and Animal Husbandry
10. Caiyun Wang: College of Veterinary Medicine, Inner Mongolia Agricultural University, China
11. Chenguang Du: College of Veterinary Medicine, Inner Mongolia Agricultural University, China
12. Haijun Li: College of Veterinary Medicine, Inner Mongolia Agricultural University, China
13. Xihe Li: College of Life Sciences, Inner Mongolia University, China
14. Guifang Cao: College of Veterinary Medicine, Inner Mongolia Agricultural University, China
15. Yongli Song: College of Life Sciences, Inner Mongolia University, China
16. Fuxiang Bao: Inner Mongolia Autonomous Region Key Laboratory of Basic Veterinary Medicine, China China
Abstract:
Early embryonic development determines fetal and adult development. However, little is known about the specific development of the early Mongolian sheep embryo. We aim to analyze the differentially expressed genes (DEGs) between 16-day embryos of two breeds of Mongolian sheep, namely, Hulunbuir short-tailed sheep and Ujumqin sheep, by mRNA sequencing. A total of 2152 DEGs were identified. Significantly DEGs include WNT5A, WNT2, AXIN2, BMP5, TGFB2, and SMAD9, which are involved in multiple signaling pathways, and genes involved in digestion and absorption and cellular differentiation, such as DPP4, ACE2, PLPP1, CD22, CD44, TEC, and JAK1. In the pathway analysis, the development of Hulunbuir short-tailed sheep 16-day embryo H(E16) highlighted pathways primarily associated with cellular interactions and organogenesis. These pathways are critical for transcriptional control during early embryonic development, especially organ formation. Genes that sophisticate organs and body mechanisms, and genes associated with protein and fat digestion, hematopoietic stem cell lineage and osteoblast differentiation, are enriched during the development of the Ujumqin sheep 16-day embryo U(E16). Our work provides important complementary reference data for the study of early embryonic development in sheep.
Page(s): 1743-1755
Published: Journal: Pakistan Journal of Zoology, Volume: 56, Issue: 4, Year: 2024
Keywords:
organogenesis , 16day embryos , Hulunbuir shorttailed sheep , Ujumqin sheep , mRNA sequencing
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