Abstract:
Thrombosis is a condition in which degradation of fibrinogen occurred by thrombin leads to formation of fibrin clots in blood. Embolism due to blood clots leads to death by causing a vascular blockage in circulatory system. Cardio vascular disorders such as, acute myocardial infarction, high blood pressure, stroke, ischemic heart disease etc., are primary causes of death. To liquefy the blood lump, thrombolytic drugs are administered. Production and purification of urokinase, a fibrinolytic enzyme and thrombolytic agent, from Oidiodendron flavum was carried out in this research work. Indigenous compost and soil samples were collected from the lawn of Biochemistry department, Islamia University of Bahawalpur. Once the sample had been processed, Oidiodendron flavum was isolated using fibrin salt basal media and inoculated on PDA (peptone dextrose-agar media) plates. Sugarcane bagasse was used as substrates for isolated fungal strain. The isolated strain (IBBB A1) of Oidiodendron flavum was used for the production, isolation and purification of urokinase. After fibrinolytic enzyme production, various purification techniques such as Ammonium sulphate precipitation, Dialysis, Ion exchange chromatography, Gel filtration chromatography was applied to get purified enzyme. The enzyme produced by the isolated strain resulted in 306 U/ml with 74.27 U/mg specific activity, 4.12 mg/ml protein, 3.81 fold purification and 83.13% recovery. Urokinase produced from isolated fungal strain exhibited activity within pH range of 7.0 to 8.0 while it gave its best performance at pH 8.0. At a temperature of 37°C, urokinase gave its highest activity. Molecular weight of Urokinase 33KD was determined by Sodium dodecyl sulphate-polyacrylamide gel electrophoresis. For this purpose growth of Oidiodendron flavum was optimized at various physical and nutritional parameters such as pH, temperature, substrate concentration and fermentation time period for obtaining maximum growth of Oidiodendron flavum and good quality urokinase with cheap cost at industrial level. Clinically, Urokinase is given to patients suffering from thrombolytic disorders. Among the plasminogen activators, Urokinase provides a superior alternative for the simple reasons of its being more potent as compared to tissue-plasminogen activator and non-antigenic by virtue of its human origin unlike streptokinase. Based on these observations, Urokinase is a strong plasminogen activator.
Page(s):
80-80
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023
Keywords:
Pakistan
,
Sugarcane bagasse
,
Fibrinolysis
,
Cardiovascular agent
,
Oidiodendron flavum
,
plasminogen activators
,
Urokinase