Variations in the Prion Protein Gene (PRNP) Sequences of Wild Punjab Urial from Pakistan | [Pakistan Journal of Zoology • 2022]

Author(s):

1. Nida Sadaqat: Center of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, Pakistan

2. Abdul Wajid: Department of Biotechnology, Virtual University of Pakistan, 1-Davis Road, Lahore, Pakistan

3. Quratul Ain: Department of Biotechnology, Virtual University of Pakistan, 1-Davis Road, Lahore, Pakistan

4. Muhammad Zia Akbar: Department of Biotechnology, Virtual University of Pakistan, 1-Davis Road, Lahore, Pakistan

5. Farkhanda Manzoor: Department of Zoology, Lahore College for Women University, Lahore, Pakistan

6. Muhammad Sarwar Khan: Center of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, Pakistan

7. Nasir Ali: Department of Molecular Biology, Virtual University of Pakistan, 1-Davis Road, Lahore, Pakistan

8. Masroor Ellahi Babar: Department of Molecular Biology, Virtual University of Pakistan, 1-Davis Road, Lahore, Pakistan

9. Tanveer Hussain: Department of Molecular Biology, Virtual University of Pakistan, 1-Davis Road, Lahore, Pakistan

Abstract:

Scrapie is a fatal neurodegenerative disease caused by the accumulation of improperly folded forms of host-encoded cellular-prion protein (PrPc) in the central nervous system (CNS) and in some peripheral tissues in sheep and goats. Scrapie resistant/susceptibility have been associated with the presence of PRNP gene polymorphisms. We analyzed the polymorphisms in PRNP gene sequences in 49 wild Punjab urial (Ovis vignei punjabiensis). Four novel amino acids polymorphisms (p.Q149E, p.Q155E, p.Y228L and p.L253F) were detected in PRNP gene. The urial was monomorphic at codon 149 and 155 and polymorphic at codon 228 and 253. These amino acid polymorphisms were combined in four alleles and six genotypes. Investigating the polymorphisms at codon 136, 154 and 171, the only genotype ARQ/ARQ was detected in Punjab urial. The PRNP gene sequences in Punjab urial showed low variability and is resemble to previous findings for wild animals. Prevalence-estimate, transmissibility, distribution, and host-range of prion disease in wild-animals remain undetermined.

Page(s): 443-446

DOI: 10.17582/journal.pjz/20191201161210

Published: Journal: Pakistan Journal of Zoology, Volume: 54, Issue: 1, Year: 2022

Keywords:

polymorphisms , Neurodegenerative disease , Prion protein gene , Wild Punjab urial , Scrapie

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