Author(s):
1. Ali Zaman:
Faculty of Veterinary and Animal Sciences, Gomal University, D.I.Khan, Pakistan
2. Faiqah Ramzan:
Faculty of Veterinary and Animal Sciences, Gomal University, D.I.Khan, Pakistan
3. Muhammad Inamullah Malik:
Faculty of Veterinary and Animal Sciences, Gomal University, D.I.Khan, Pakistan
Abstract:
Globally Avian Influenza is one of the major etiological factors responsible for mortality in birds and responsible for major economic losses up to 89%.It have a great serious impact on public health as well.This study includes seroprevalence and Molecular Characterization of Avian Influenza strain H9N2 during the year 2020-2021.A total50 number of samples were collected from five tehsils of District Dera Ismail Khan with different environmentalcircumstances. In whole District, the overall seroprevalence of Avian Influenza Strain H9N2 was 24%while in five Tehsils of District Dera Ismail Khan was 30% in Darabin, 10% in Dera Ismail Khan, 20% in Kulachi, 20% in Paharpur and 40% in Tehsil Parova. The Seroprevelence in Aseel was significantly lower than the previous studies in the broiler chickens. It was may be due to high resistance in Aseel or may be due to managemental practices. Significant lowest seroprevalence was observed in urban and city areatehsil Dera Ismail Khan (10%) and highest inrural and backward tehsil Parova (40%). In hot months of summer season, significant lower cases were noticed in comparison to winter. Seroprevalence was significantly lower in vaccinated and birds kept in good conditions. Furthermore it was come to conclude that the seroprevalence was more in sick birds than the healthy ones. Theconfirmation of positive samples of H9N2 was done with the help of PCR.Then phylogenic study of positive samples was conducted. Using PCR assay Targeting hemagglutinin gene (HA) 808bp bands were amplified. All strains of AIV H9 were clustered nearer to the already reported Pakistani local strains in the 2015-19. AIV H9N2 strains of the study representedoverall 11-13% genomic distance to B1 strains sub-lineages reported inIran India and Pakistan. Same wise a 13.5-18% genomic distance was reported among G1_Mideast_Group AViruses lineage followed by 20.5-24% among Y9280-like lineage viruses and 26-29% among Y439/97-like viruses lineage. .
Page(s):
228-228
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023
Keywords:
Molecular characterization
,
Recombinant H9n2 Influenza Virus
,
Aseel Chikcken
References:
References are not available for this document.
Citations
Citations are not available for this document.