The family Euphorbiaceae comprises 2000 species and is listed as third among the largest flowering family. Euphorbia is used in traditional treatment for various diseases, including dengue fever, dysentery, diarrhea, and anemia. This review aims to collect data from various published literature sources for quick and efective cultivation of Euphorbia species through tissue culture and documentation of potent secondary metabolites obtained from diferent cultures of Euphorbia and its manifold pharmacological activities from various parts extracts. The data for this review were systematically collected from diferent scientific databases, including Google Scholar, Science Direct, PubMed, and published literature. Diferent secondary metabolites have been reported from the in votro culture of Euphorbia containing anthocyanin, saponins, tannins, sterols, flavonoids, glycosides, diterpenes, and sesquiterpene. The essential oils from extractions of the Euphorbia genus embraced about 80 active phytochemical constituents. The extracts and compounds exhibited diferent pharmacological activities, including hepato-protective, anti-fungal, anti-bacterial, and anti-cancer. Besides the pharmaceutical and importance of the genus Euphorbia, this report also described the methodologies of explant cultures, in-vitro production of biologically active compounds, and vital phytochemicals extraction from various parts of Euphorbia. Therefore, there is great attention for in-vivo studies on Euphorbia to further investigate and confirm their therapeutic efects for safe and efective medical use. The in-vitro cultivation technique needs further development, either in bioreactors or temporary immersions and shakes flasks to obtain vigorous sprouts of Euphorbia.