Abstract:
Elevated levels of reactive oxygen species (ROS) pose severe threats to the intracellular environment in terms of oxidative stress, and their continuous overproduction causes various clinical disorders. Antioxidant compounds combat oxidative stress in cells and are therefore useful in the management of various clinical disorders. Plants are an important source of bioactive compounds, especially mucilage containing polysaccharides have been extracted from plants and studied for various biomedical applications. Lepidium sativum, commonly known as garden cress, whose seeds release mucilage upon soaking in water. The aim of the current study was to extract polysaccharides via size exclusion chromatography (SEC) from the seed coat mucilage of garden cress and to evaluate the bioactive fraction(s). The mucilage was extracted by soaking cress seeds in distilled water. The collected mucilage was then precipitated with75% ethanol. The ethanol precipitated fraction was further fractionated through Bio-Gel P-10 (90 µm-180 µm). A total of 60 (2 ml each) fractions were collected tested for the presence of carbohydrates. The results revealed the presence of pentoses, hexoses, uronic acids and unsaturated uronic acids. Among all the screened samples for their antioxidant potential through DPPH assay, significant EC50 value was shown by fraction F23 as its EC50 was 37.93µg/mL, followed by fraction F52 (58.04 µg/mL) and fraction F53 (66.04µg/mL), respectively. Selected SEC fractions of ethanol precipitated polysaccharides extracted from cress seed coat mucilage were then screened forH2O2 radical scavenging activity. Each fraction was screened at three different concentrations, including 50, 100 and 200 µg/mL. The results obtained showed that fraction F53 was the most effective fraction at 200 µg/mL as it scavenged the H2O`2 free radical by 81.9%±0.24, followed by fraction F18 (76.54%±0.65)and fraction F23 (76.4%±1.96). In vitro lipid peroxidation assay was performed on mice tissue samples where F52 fraction was the most effective one havingIC50 value of 34.17 µg/mL, followed by F20 having IC50 value of 57.18 µg/mL.Our results concluded that water-soluble polysaccharides extracted from cress seed coat mucilage can be used as potential and effective natural antioxidant compounds.
Page(s):
131-131
DOI:
DOI not available
Published:
Journal: 1st International Conference on "Recent Advances in Green Biotechnology and Climate Resilience", September 15-16, 2025, Volume: 1, Issue: 1, Year: 2025
Keywords:
bioactive compounds
,
endophytes
,
exopolysaccharides
,
Wheat plant