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Uncovering the Role of Ribosomal Protein L8 in Milk Fat Synthesis Mechanisms in Yak Mammary Epithelial Cells
Author(s):
1. Nan Jiang: State Key Laboratory of Hulless and Yak Germplasm Resources and Genetic Improvement, Lhasa, Tibet, 850000, China; Institute of Animal Husbandry and Veterinary, Tibet Autonomous Regional Academy of Agricultural Sciences, Lhasa, Tibet,850000, China.
2. Chaochao Luo: College of Life Sciences, Shihezi University,Shihezi, Xinjiang 832003,P.R. China
3. Mingying Shao: Rural Revitalization College, Hainan College of Vocation and Technique Haikou,Hainan, 570100,China
4. Ziping Zheng: Rural Revitalization College, Hainan College of Vocation and Technique Haikou,Hainan, 570100,China
5. Qudrat Ullah: Faculty of Veterinary and Animal Sciences, University of Agriculture,Dera Ismail Khan,Pakistan
6. Muhammad Zahoor Khan: State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, Beijing, China; Faculty of Veterinary and Animal Sciences, University of Agriculture, Dera Ismail Khan, Pakistan.
7. Guangming Sun: Institute of Animal Husbandry and Veterinary, Tibet Autonomous Regional Academy of Agricultural Sciences, Lhasa,Tibet,850000,China
8. Dun-Zhu Luosang: Institute of Animal Husbandry and Veterinary, Tibet Autonomous Regional Academy of Agricultural Sciences, Lhasa,Tibet,850000,China
9. Rubina Mushtaq: Institute of Molecular Biology and Biotechnology, The University of Lahore,Lahore,Pakistan
10. Yulin Ma: State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University,Beijing,China
11. Wang-Dui Basang: State Key Laboratory of Hulless and Yak Germplasm Resources and Genetic Improvement, Lhasa, Tibet, 850000, China; Institute of Animal Husbandry and Veterinary, Tibet Autonomous Regional Academy of Agricultural Sciences, Lhasa, Tibet,850000, China.
Abstract:
The fat content in yak milk is higher than that in dairy cows, but the molecular mechanisms responsible for milk fat synthesis in yaks are still unclear. This study examined the regulatory mechanism of milk fat synthesis in yak mammary epithelial cells (YMECs) by investigating the role of Ribosomal protein L8 (RPL8) in the mTORC1-SREBP1 signaling pathways. The results showed that over-expression or inhibition of RPL8 had a significant effect on triglyceride (TG) secretion, which also affected the sterol regulatory element-binding protein 1 (SREBP1) pathway. Similarly, the intervention of SREBP1 revealed that RPL8 promoted TG secretion through the SREBP1 pathway. Additionally, the study found that over-expression or inhibition of RPL8 regulated the signaling activity of the mammalian target of rapamycin complex 1 (mTORC1), which promoted the SREBP1 signaling pathway through mTORC1. Further examination by over-expressing or inhibiting SREBP1 or mTOR showed that mTOR promoted TG secretion through the mTORC1-SREBP1 signaling pathway. Moreover, YMECs treated with palmitic acid showed increased expression of RPL8, mTOR, and SREBP1, as well as increased TG secretion. Overall, the study’s findings provide insights into the underlying mechanisms of milk fat synthesis in yaks, and suggest that the RPL8 gene and mTORC1-SREBP1 signaling pathways could serve as potential genetic markers for milk fat synthesis in yak mammary glands.
Page(s): 2263-2278
Published: Journal: Pakistan Journal of Zoology, Volume: 56, Issue: 5, Year: 2024
Keywords:
milk fat , YMECs , RPL8 , mTORC1 , SREBP1
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