A study on the optimization of physico-chemical conditions for the growth of Pasteurella multocida under in vitro. | [Journal of Agriculture and Social Sciences • 2008]

Author(s):

1. Atta Hussain Shah: Department of Dairy Technology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam, Pakistan

2. A. A. Kamboh: Veterinary Microbiology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam, Pakistan

3. N. Rajput: Poultry Husbandry, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam, Pakistan

4. N. A. Korejo: Veterinary Medicine, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam Pakistan

Abstract:

A study on physico-chemical growth conditions of Pasteurella multocida was carried-out at Vaccine Production Unit, Tandojam, Sindh, Pakistan. The organism was grown in brain heart infusion medium containing different physical and chemical agents. A suitable temperature for its growth was recorded in between 35°C and 40°C, whereas poor activities were observed at below 30°C and the organism did not grew well at 50°C and above. The influence of shaking in terms of different rotation per minute (rpm) was also determined during investigation. Under shaking conditions (500 rpm for 24 h) good growth was achieved. Positive correlation of shaking and growth at 500 rpm for 24 h was observed. The influence of pH was recorded during the experiment. No growth occurred at pH 0.5 and 10.0 but best growth was obtained at pH ranged from 7.0-8.0. In addition to that, use of orange juice as supplement failed to support the growth, whereas apple juice (15%) showed positive influence on the growth of P. multocida. Moreover, different concentrations of succrose were used but only 1.2% supported the growth. As succrose was costly, it was replaced with table sugar at various concentrations, out of which only 0.6% well supported the growth.

Page(s): 176-179

DOI: DOI not available

Published: Journal: Journal of Agriculture and Social Sciences, Volume: 4, Issue: 4, Year: 2008

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