Abstract:
Current therapeutic options for various human ailments have faced challenges such as resistance, low efficacy, high costs, and increasing market demands, driving the search for novel, cost-effective natural alternatives. Nanoparticles, particularly those synthesized through green methods, have emerged as pivotal in biotechnology and therapeutics due to their high biocompatibility and potent bioactivities, including antimicrobial, anti-inflammatory, antioxidant, and anticancer properties. This study focused on the biosynthesis of silver nanoparticles from endophytic exo polysaccharides (EPS) of Triticum aestivum. The EPS was extracted and precipitated using ethanol, followed by lyophilization. The presence of polysaccharides was confirmed through the phenol-sulfuric acid (PSA) method. Silver nanoparticles (AgNPs) were synthesized by treating the polysaccharide extracts with the respective metallic salts. The biosynthesis process was optimized using UV-visible spectrophotometry and characterized using FT-IR techniques to confirm the formation and stability of the nanoparticles. The in vitro potential of the synthesized nanoparticles was assessed through a-amylase and a-glycosidase inhibition assays. The results revealed that AgNPs significantly inhibited a-amylase activity, with an IC50 of 265.75 µg/mL, followed by EPS (357.18µg/mL). NiO NPs, on the other hand, were found to significantly inhibit a-glycosidase, with an IC50 of 441.87 µg/mL, followed by EPS (590.68 µg/mL).These findings underscore the potential of biosynthesized MNPs as effective natural therapeutics, offering a promising alternative to conventional treatments, with significant implications for the development of cost-effective and biocompatible therapeutic agents.
Page(s):
118-118
DOI:
DOI not available
Published:
Journal: 1st International Conference on "Recent Advances in Green Biotechnology and Climate Resilience", September 15-16, 2025, Volume: 1, Issue: 1, Year: 2025
Keywords:
Green synthesis
,
Silver nanoparticles
,
Triticum Aestivum
,
Endophytic exopolysaccharides