Thioglycol in extender improves the post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa. | [Journal of Animal and Plant sciences • 2014]

Author(s):

1. M. S. Ansari: Department of Zoology, University of Gujrat, Gujrat, Pakistan

2. R. Iqbal: Department of Zoology, University of Gujrat, Gujrat, Pakistan

3. B. A. Rakha: Department of Wildlife Management, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan

4. S. M. H. Andrabi: Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, 46300, Pakistan

5. N. Ullah: Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, 46300, Pakistan

6. S. Akhter: Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, 46300, Pakistan

7. W. V. Holt: Institute of Zoology, Zoological Society of London, Regent’s Park, London NW1 4RY, UK

Abstract:

This study was designed to evaluate the effect of thioglycol in extender on post-thaw quality of Nili-Ravi buffalo (Bubalus bubalis) bull spermatozoa. Semen from three buffalo bulls was collected at weekly intervals (three replicates; two ejaculates per bull per replicate) and qualifying ejaculates (n=18) were cryopreserved in extenders containing thioglycol at 0.0 or 0.5 or 1.0mM. Sperm motility (%; visually), plasma membrane integrity, (%;supravital hypo-osmotic swelling test), viability (%;dual staining technique using Trypan blue and Giemsa stains) and DNA integrity (%;acridine orange test) was assessed at 0, 2 and 4 hour post-thaw. Sperm motility, plasma membrane integrity and viability of buffalo bull spermatozoa were improved in a dose dependent manner with the supplementation of thioglycol at 0.5 mM and 1.0mM compared control. Sperm DNA integrity was similar (P>0.05) in extenders containing 0.5 mM and 1 mM thioglycol that remained higher (P

Page(s): 1256-1259

DOI: DOI not available

Published: Journal: Journal of Animal and Plant sciences, Volume: 24, Issue: 4, Year: 2014

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