Tomato leaf curl Palampur virus; an otherwise bipartite begomovirus infects the chili crop in association with betasatellite | [Pakistan Journal of Agricultural Sciences • 2023]

Author(s):

1. Zonara Zafar: Virology lab, Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture,Faisalabad,Pakistan

2. Muhammad Shah Nawaz-ul-Rehman: Virology lab, Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture,Faisalabad,Pakistan

3. Muhammad Mubin: Virology lab, Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture,Faisalabad,Pakistan

4. Amer Jamil: Department of Biochemistry, University of Agriculture,Faisalabad,Pakistan

Abstract:

The most devastating pathogens of crop plants in the world are single-standard DNA viruses (Family, Geminiviridae). A vast majority of these plants are infected by the viruses belonging to the genus Begomoviruses. Chili is widely cultivated all over the world and plays an important role in the commercial sector. However, chili production in the Indian subcontinent is affected by chili leaf curl disease (ChiLCD) where it can cause a 100% loss to the crop. The current research was conducted to comprehend the begomovirus disease complex causing leaf curling disease of chili crop in Pakistan. Chili fields were surveyed for collecting infected plants with symptoms of leaf curling and stunted growth. Genomic DNA was extracted and full-length virus molecule and related satellites were cloned from infected plants using a combination of cloning techniques PCR and rolling circle amplification. A diagnostic PCR for the begomoviruses and satellites identified the presence of a well-known tomato leaf-curl New Delhi virus in the majority of the samples. However, two symptomatic plant samples showed the infection with a different begomovirus. The sequence and phylogenetic analysis of the virus molecules isolated from these two samples revealed the presence of tomato leaf curl Palampur virus (ToLCuPaV) DNA-A and an associated chili leaf curl betasatellite (ChiLCuB) in Pakistan. The nucleotide identity analysis further revealed that the Palampur isolate of DNA-A of ToLCuPaV isolated from chili in Pakistan is 97% identical to the Indian isolate (GenBank Accession-GU253914). The unique character of this isolate was the absence of DNA-B and the presence of satellite molecule. The attempts made to amplify DNA-B component using PCR or RCA were not successful. Infectious construct of ToLCuPaV was made and tested for infectivity in Nicotiana benthamiana plants using agro-inoculation with or without the ChiLCuB. The infectivity experiments suggested that DNA-A of ToLCuPaV alone was able to induce curling of leaves in N. benthamiana. Severe leaf curling symptoms were observed when N. benthamiana plants were co-inoculated with ToLCuPaV and ChiLCuB. Thus, it is concluded that betasatellite plays a critical role in the development of severe disease symptoms in tobacco plants and ToLCuPaV reported on chili plants is associated with ChiLCB instead of DNA-B component.

Page(s): 117-124

DOI: 10.21162/PAKJAS/23.124

Published: Journal: Pakistan Journal of Agricultural Sciences, Volume: 60, Issue: 1, Year: 2023

Keywords:

Diversity , Begomovirus , betasatellite , geminivirus , Infectivity analysis

References:

References are not available for this document.

Citations

Citations are not available for this document.

Citations

Downloads

Views