Trace Elements Optimization for Production of Fibrinolytic Protein from Wild and Mutant Streptococcal Strains | [Journal of Innovative Sciences • 2022]

Author(s):

1. Ghulam Akbar: Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan

2. Muhammad Anjum Zia: Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan

3. Amer Jamil: Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan

4. Faiz Ahmad Joyia: Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad, Pakistan

Abstract:

Streptokinase is a fibrinolytic enzyme produced from various bacterial species especially beta hemolytic Streptococci. Among these beta hemolytic strains the Streptococcus equisimilis is being used for industrial streptokinase production. It has 414 amino acids globular chain with 47000 D molecular weight. hTe fibrinolytic drug is comparatively cheap as compared to all other fibrinolytic drugs. It is the drug of choice in all low income nations and developing countries. The mortality rate with cardiovascular diseases is 85% in less developed countries and 75% prevalence is in women. The wild and mutant strains of Streptococcus mutans were used for this trace element optimization study. There are some salts like KH 2PO4, K2HPO4, NaHCO3, CaCO3, CH3COONa.3H2O and FeSO4. 7H2O have been optimized for the production of SK. The K 2HPO4 has shown the highest enzymatic activity with UV-Et mutant 680.0 UmL-1 and with CaCO3 UV-Et mutant has been found to give the relatively lowest enzyme activity 652.01UmL-1. While UV-mutant has shown maximum enzyme activity 275 UmL-1 with the addition of K HPO4 and this mutant shown minimum 57 2 UmL-1 with the addition of FeSO4. 7H2O. he parental strain expressed maximum enzyme activity with 108 UmL-1 FeSO4 minimum enzymatic activity 5 UmL-1 with the addition of CH3COONa.3H2O.

Page(s): 301-310

DOI: 10.17582/journal.jis/2022/8.2.301.310

Published: Journal: Journal of Innovative Sciences, Volume: 8, Issue: 2, Year: 2022

Keywords:

optimization , trace elements , Streptokinase , Enzyme production , Fibrinolytic

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