Author(s):
1. Mishal Subhan:
Department of Microbiology and Molecular Genetics, The women University Multan, Pakistan
2. Rani Faryal:
Department of Microbiology, Quaid-I-Azam University, Islamabad, Pakistan
3. Ian Macreadie:
School of Science, Royal Melbourne Institute of Technology University, RMIT, Bundoora,
Melbourne Australia
Abstract:
Lovastatin is produced by fungus Aspergillus terreus during a polyketide pathway. This not only reduces the cholesterol levels but also acts as a precursor molecule for the synthesis of simvastatin, the widely prescribed statins for the prevention of strokes and heart disease. In this study, Corymbia maculata leaves were tested as a novel substrate and carbon source for the growth of a newly isolated of A. terreus and a lovastatin-resistant mutant strain of A. terreus to produce lovastatin. Corymbia maculata also known as spotted gum is well utilised by forest industries as a source of timber because of its smooth texture, high strength, and durability. However, these leaves are a major waste product in forest industry. The aim of present study was to discover the forestry waste for the production of lovastatin and generation of lovastatin resistant mutant. The aim of present study was to discover the forestry waste for the production of lovastatin and generation of lovastatin resistant mutants for its increased productivity and enhanced levels. Growth of Aspergillus terreus on Corymbia maculata leaves, in solid-state fermentation (SSF) resulted in the production of lovastatin. Fermentation of media using fresh green leaves of Corymbia maculata produced more lovastatin (4.9 mg g-1), than the sun-dried brown leaves (3.2 mg g-1). Levels of lovastatin were further increased by the lovastatinresistant strain of Aspergillus terreus (Lvs-r), which produced twice the amount of the parental wild type strain. The production of lovastatin was confirmed by HPLC and LC-MS/MS studies for its increased productivity and enhanced levels.
Page(s):
280-280
DOI:
DOI not available
Published:
Journal: Abstract Book on 9th Annual Neuroscience Conference (ANC-23) August 12-13, 2023 , Volume: 0, Issue: 0, Year: 2023
Keywords:
fermentation
,
Lovastatin
,
Lovastatin
,
Lovastatin
,
Lovastatin
,
Lovastatin
,
Lovastatin
,
Lovastatin
,
Lovastatin
,
Lovastatin
,
Lovastatin
,
Lovastatin
,
Corymbia maculate
,
fungal
,
A terreus
References:
References are not available for this document.
Citations
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