A biolistic approach for the production of transgenic plants using emryogenic tissue in Pinus kesiya Royle ex. Gord (khasi pine). | [Biotechnology • 2007]

Author(s):

1. Ravindra B. Malabadi: Division of Plant Biotechnology, Department of Botany, Kamatak University, Pavate nagar, Dharwad, Karnataka state, India

2. K. Nataraja: Division of Plant Biotechnology, Department of Botany, Kamatak University, Pavate nagar, Dharwad, Karnataka state, India

Abstract:

Pinus kesiya Royle ex. Gord (Khasi pine) is an economically important early successional pine species predominant in the North-East- India. Some genotypes have tremendous biomass potential and oleoresin prospects. This study was aimed at developing a protocol for the genetic transformation of P. kesiya embryogenic tissue. This was achieved via the introduction of a bar-GUS cassette under the control of the ubiquitin promoter, through biolistic transfer. Expression of positive histochemical GUS activity (41%) in the bombarded embryogenic tissue was observed. PCR analysis of bar transgenes (46%) transformation efficiency indicated successful genetic modifications of P. kesiya embryogenic tissue by the pAHC25 plasmid. This is the first successful report of genetic transformation in P. kesiya indicating that Khasi pine is amenable to gene transfer.

Page(s): 86-92

DOI: DOI not available

Published: Journal: Biotechnology, Volume: 6, Issue: 1, Year: 2007

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