Abstract:
The excessive formation of Reactive Oxygen species (ROS) due to any abnormal stimuli impart deleterious effects on the internal cellular environment. Natural products being rich in various bioactive compounds used to provide broad range of protection against these reactive oxygen species. Phoenix dactylifera var. Ajwa, is amongst the bestdietary sources of natural compounds which is equally beneficial, both nutritionally and economically. Because of its exceptional antioxidant properties, it grabs the attention of nutritionist and natural product scientist to further probe on the spectrum of efficacy against the cellular oxidative damages. Thisresearch aimed to study the combine effect of Ajwa date fruit and seed extracts on total polyphenol content and free radical scavenging activity through various in-vitro and ex-vivo antioxidant assays with identification of bioactive compounds through GCMS. Inthe present study the total phenolic and flavonoid contents were found to be 993mg-5020mg and 163mg-907mg as GAE/100g and RE/100g respectively. DPPH scavenging assay was represented in terms of Ascorbic Acid and Trolox equivalent, i.e., 4.293-26.910mM AAE/100g and 4.637-28.317mM TEAC/100g respectively. While ABTS decolorizing assay was observed in the range of 9.61-29.18mM TEAC/100g. Moreover, Beta-carotene assay revealed that combination extract prevented the oxidation of beta-carotene in the range of 27.76-69.14%. Oxidative hemolysis inhibition assay on HRBCs showed that methanolic extract was more potent followed by ethyl acetate extract, i.e., 93-94% inhibition has been shown. GCMS studies of extract mixture revealed several importantchemical constituents such as Methyl hexadecanoate (42%), Hexadecanoic acid (32%), Ethyl Hexadecanoate (32%), Methyl (E)-9-dodecenoate (30%), Methyl cis-11- octadecenoate (56%), Methyl 9,12-octadecadienoate (28%), Methyl (E)-9-Octadecenoate (67%), (Z,Z)-9,12-Octadecadienoic acid (34%), Cholestan-22(26)-epoxy-3,16-dione (9%), Ethyl isoallocholate (5%), Ergost-5-en-3-ol, acetate, (3ß,24R) (7%), Stigmastan-3,5-diene (19%), a- Tocopherol (27%), Lup-20(29)-en-3-one (3%), Stigmast-4-en-3-one (17%) Stigmasta-5, 22-dien-3- ol, acetate-(3ß) (11%), Stigmast-5-en-3-ol, (3ß,24S)- (35%) and Stigmast-5-en-3-ol, Oleate (2%). In addition, in the above listed compounds found in Phoenix dactyliferasome of them showed bioactivity as per literature, which might be further explored to work out for their prominent role in combating cardiovascular& other disorders. Further, such exploration may also be useful for the nutraceutical and pharmaceutical industries to develop commercial products based on natural compounds for the maintenance of cardiovascular health &various ailments.
Page(s):
304-304
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
antioxidant
,
antioxidant
,
antioxidant
,
Polyphenols
,
Ajwa
,
GCMS