Abstract:
Chia seeds (Salvia hispanica L.), a natural source of mucilage polysaccharides, exhibit remarkable biological potential with demonstrated antioxidant, prebiotic, and antimicrobial activities. This study presents the green synthesis of iron nanoparticles using chia seed polysaccharides as both a reducing and stabilizing agent. The biosynthesized iron nanoparticles were characterized through UV visible spectroscopy, X-ray diffraction (XRD), scanning electron microscopy(SEM), and X-ray diffraction analysis, confirming successful nanoparticle formation. The antibacterial potential of Fe-NPs was assessed against five human pathogenic strains i.e Staphylococcus aureus, Salmonella typhi, Shigellaspp., Klebsiella pneumoniae, and Salmonella spp using agar well diffusion, microbial biofilm inhibition, and minimal inhibitory concentration (MIC)assays. Results demonstrated that Fe-NPs at 100 µg/mL produced inhibition zones of 8.78 mm against S. aureus and 8.7 mm against S. typhi, compared to7.73 mm for chia seed polysaccharides alone against S. aureus. The microbial biofilm inhibition assay revealed an IC50 of 15.67 µg/mL for Fe-NPs, while chia seed polysaccharides exhibited an IC50 of 14.21 µg/mL against Klebsiella pneumoniae. MIC assays indicated that Fe-NPs effectively inhibited the growthof S. aureus at a concentration of 28.12 µg/mL, with higher concentrations required for Shigella spp. (57.59 µg/mL), Klebsiella pneumoniae (87.49µg/mL), and Salmonella spp. (54.22 µg/mL). These findings underscore the potent antibacterial activity of chia polysaccharide-based Fe-NPs, highlighting their potential as a natural, eco-friendly alternative for biomedical applications in combating bacterial infections.
Page(s):
129-129
DOI:
DOI not available
Published:
Journal: 1st International Conference on "Recent Advances in Green Biotechnology and Climate Resilience", September 15-16, 2025, Volume: 1, Issue: 1, Year: 2025
Keywords:
antibacterial
,
mucilage
,
Polysaccharides
,
Iron oxide nanoparticles
,
Chia Seeds