BOT-1928: Biogenic synthesis of Zinc Oxide Nanoparticles using Thymus linearis Extract, Characterization and Evaluation of Different Biological Activities | 4th International Conference of Sciences “Revamped Scientific Outlook of 21st Century, 2025” , November 12,2025 2025

Author(s)

Farishta Zarshan Department of Botany, Bacha Khan University,Charsadda, 24420, KPK,Pakistan

Javed Iqbal Department of Botany, Bacha Khan University,Charsadda, 24420, KPK,Pakistan

Banzeer Ahsan Abbasi Department of Botany, Rawalpindi Women University,6th Road, Satellite Town, Rawalpindi 46300,Pakistan

Madiha Imtiaz Department of Botany, Bacha Khan University,Charsadda, 24420, KPK,Pakistan

Syeda Anber Zahra Institute of Biomaterial & Biomedical, Shanghai Zhongqiao Vocational and Technical University.,Shangai,China

Abstract

Zinc oxide nanoparticles possess unique and fascinating properties and have gained the significant attention of scientific community due to their multifaceted applications in different commercial areas. However, the traditional methods for the preparation of ZnO-NPs are linked with environmental and health hazards. To overcome these problems and challenges, the scientists have developed an alternate route for the synthesis of multifunctional nanoparticles known as green synthesis, which uses different natural resources and bio-templates. In the present research study, ZnONPs have been prepared using Thymus linearis (TL) aqueous extract as both reducing and stabilizing agents. Several analytical methods were employed to validate the synthesis of as-prepared ZnONPs. FTIR, SEM, EDX. XRD analysis was done to understand the crystalline nature of biogenic ZnONPs and the size was calculated about 30.39 nm. Furthermore, the TL-ZnONPs were examined for multifaceted biological activities. The antibacterial properties were demonstrated using different bacterial strains revealing significant antibacterial potential. The maximum resistance was observed in case of Enterococcus faecalis (MIC 125µg/ml), however Staphylococcus saprophyticus (MIC 31.25µg/ml) was found to be the most sensitive among all strains. The fungicidal properties were demonstrated using different fungal strains. The maximum resistance against all doses was observed in case of Aspergillus flavus (MIC 125 µg/ml). However, Aspergillus niger (MIC 31.25µg/ml) was found to be the most sensitive among all strains. Further, our T. linearis-ZnO nanoparticles revealed excellent cytotoxic potential against nascent brine shrimps larva’s (IC50 34.16 µg/ml). In summary, our NPs have shown strong biological potential against different assays. In future we recommend further biological activities using in vitro and in vivo models.

Publication Details

Page(s) 52-52

DOI DOI not available

Published Journal: 4th International Conference of Sciences “Revamped Scientific Outlook of 21st Century, 2025” , November 12,2025, Volume: 1, Issue: 1, Year: 2025

Keywords

antibacterial Antifungal Zinc oxide nanoparticles Thymus linearis Cytotoxic potential

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