An in vivo and in vitro analysis of free radical scavenging potential possessed by Desmodium gangeticum chloroform root extract: Interpretation by GSMS. | [Pakistan Journal of Pharmaceutical Sciences • 2012]

Author(s):

1. Shyam Srivats: Department of Biotechnology, SASTRA University, Thirumalaisamudram, India

2. Gomathi Ramakrishnan: Department of Biotechnology, SASTRA University, Thirumalaisamudram, India

3. Jose Paddikkala: Department of Plant Biotechnology, Amala Cancer Research Center, Amalanagar, Trichur, Kerala, India

4. Gino Andrew Kurian: Medical Biochemistry, School of Chemical and Biotechnology, SASTRA University, Thirumalaisamudram, Thanjavur, Tamil Nadu, India

Abstract:

In the present study, the effect of DG chloroform root extract was assessed on isolated rat heart and in-vitroantioxidant models. Ischemia reperfusion injury was experimentally induced by using Langendroff apparatus. The free radical scavenging potential was studied in vitro by using different antioxidant models such as DPPH, super oxide scavenging activity, hydroxide scavenging activity and nitric oxide scavenging activity. Both experimental approaches not only substantiate its antioxidant potential but also the cardio-protection imparted by the extract. The cardiostimulatory effects were investigated for the extract by treating it as a pre-conditioning agent against myocardial ischemia reperfusion injury. The improved antioxidant status of the myocardium indirectly predicts reduced oxidative stress mediated by ischemic reperfusion with evident reduction of infarct size determined by cardiac marker protein. These findings indicate that DG chloroform root extract may possess therapeutic potential against ischemia reperfusion injury.

Page(s): 27-34

DOI: DOI not available

Published: Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 25, Issue: 1, Year: 2012

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