Abstract:
The purpose of this study was to investigate the synthesis, characterization, and antifungal efficacy of silver nanoparticles of Amaranthus viridis L. against Botrytis cinerea Pers. Silver nanoparticles were formed from a plant extract using methanol and examined using Scanning Electron Microscopy (SEM), UV-Visible spectroscopy, X-Ray diffraction analysis (XRD), and particle size analysis. UV-Visible analysis indicated maximum absorption for AgNPs synthesized from A. viridis L. at 425 nm and an average particle size was observed as 1.2 nm respectively. FTIR analysis confirmed the presence of hydroxyl, Aldehydes and carboxylic groups. Crystalline nature was confirmed by XRD analysis of silver nanoparticles and spherical to oval shape was observed by SEM. Silver nanoparticles (1, 1.5, 2, 2.5, 3, 3.5%) were tested against B. cinerea biomass for their antifungal efficacy. A. viridis silver nanoparticles caused growth retardation of fungi by 51% when applied in 3% concentrations. In the following step, different organic fractions, including chloroform, n-butanol, n-hexane, and ethyl acetate, were prepared from plant methanolic extract of A. viridis. The antifungal activity of these fractions was also evaluated, and B. cinerea biomass was 56% inhibited by the ethyl- acetate fraction. 15 compounds were detected using gas chromatography-mass spectrometry (GC-MS) namely Hexadecanoic acid, sesquiterpenoids, 9,12-octadecadienoic acid methyl ester, triterpene, methyl ester, Methyl stearate, diterpene, Phenols, siloxane and stigmastanes, Hydrocardons. These findings proved that A. viridus L. silver nanoparticles are highly effective against B. cinerea.
Page(s):
142-142
DOI:
DOI not available
Published:
Journal: Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 , Volume: 0, Issue: 0, Year: 2023
Keywords:
Characterization
,
antifungal activity
,
Silver nanoparticles
,
Botrytis cinerea