UPLC separation of phytoalexins from Phaseolus vulgaris treated with elicitor from Colletotrichum lindemuthianum. | [Asian Journal of Scientific Research • 2008]

Author(s):

1. Fatima-Bi: Department of Chemistry, Royal Holloway and Bed ford New College, University of London, Surrey TW20 OEX, UK

2. Muhammad Arman: Department of Chemistry, Royal Holloway and Bed ford New College, University of London, Surrey TW20 OEX, UK

3. Seema Iqbal: Department of Chemistry, Royal Holloway and Bed ford New College, University of London, Surrey TW20 OEX, UK

Abstract:

Induced secondary metabolites produced in Phaseolus vulgaris tissues on treatment with partially purified elicitor preparations PPF- I and PPF-2 of Colletotrichum lindemuthianum were extracted with 95% alcohol and analyzed by high performance liquid chromatography. Separation was achieved on a reverse phase column on spherisorb 5 ODS 2 by gradient elution. Significant differences were observed in timing and level of induced secondary metabolites in response to various dilutions of elicitor. Hundred micro gram glucose eq mL-` elicitor concentrations of both fractions was found effective and induced high level of metabolites at 24 h of incubation of treated tissues of Phaseolus vulgaris.

Page(s): 160-165

DOI: DOI not available

Published: Journal: Asian Journal of Scientific Research, Volume: 1, Issue: 2, Year: 2008

Keywords:

Keywords are not available for this article.

References:

References are not available for this document.

Citations

Citations are not available for this document.

Citations

Downloads

Views