Abstract:
After transcription, processing and transport to the cytoplasm the mRNA may become a subject of various factors which activate or repress its translation. This work aimed to determine the start time of translation of mRNAs stored in the egg or transcribed from the zygote genome. The polyribosome/ informosomes ratio served as a criterion of mRNA involvement in translation. Here we used cesium chloride density gradient centrifugation to study the distribution of Xwnt-11, Xnot-2, Xbra and Xgsc mRNAs between polyribosomes and informosomes in the eggs and early embryos of Xenopus laevis and Rana temporaria. Present results gave evidence that specific mRNAs which encode proteins participating in dorsoventral differentiation, follow individual dynamics of activation and inactivation.
Page(s):
115-120
DOI:
DOI not available
Published:
Journal: Journal of Biological Sciences, Volume: 6, Issue: 1, Year: 2006