Abstract:
WRKY is an important group of transcription factors (TrFs) gene family and was identified primarily in plants. WRKY TrFs play vital roles in modulating gene expression during several biotic and abiotic stresses, senescence and different hormone responses. The DNA-binding domain of these proteins is a conserved hepta-peptide sequence WRKYGQK. Although the Mandarin Citrus (Citrus reticulata) genome has been published but functional studies are still necessary to understand Mandarin Citrus. The mining of C. reticulata genome identify 46 CrWRKY genes that are classified into three main groups (G1-G3) with five subclasses (IIa-IIe) in G2, and all were present on 29 different scaffolds representing numerous segmental duplication (100%) events. Through multiple sequence analysis it is predicted that WRKYGQK domain and metal chelating zinc-finger motif C2H2 is present in 45 genes while WRKYGQK domain is replaced with WRKYGKK only in CrWRKY20.The study identified light responses in CrWRKY5, CrWRKY6, CrWRKY9 and CrWRKY22; auxin responsive in CrWRKY6, CrWRKY9 and CrWRKY16; methyl-jasmonate responsive in CrWRKY9, CrWRKY18, CrWRKY35 and CrWRKY42; CrWRKY2, CrWRKY6 and CrWRKY9 are involved in defense and drought; CrWRKY1, CrWRKY9, CrWRKY15 and CrWRKY16 play role in cold and some CrWRKY genes played its role in wounding and GA3 responsiveness. Overall, 28 CrWRKY genes respond to both intimating interconnection between abiotic stresses and hormone signals for plant growth. Comparative study of syntenic region indicated a significant level of collinearity between C. reticulata-C. grandis come lateral the dicot (C. clementine, C. sinensis, C. medica, Arabidopsis thalian, C. ichangensis) and monocot (O. sativa) species. The aim of present review is to focus on the potential application of CrWRKY TrFs in plants to regulate various stresses and signaling pathways mediating expression of WRKY genes are also illustrated.
Page(s):
112-112
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023
Keywords:
Citrus reticulata
,
transcription factor
,
Expression pattern
,
WRKY gene family
,
Genomewide