A micropropagation system for cloning of hemp (Cannabis sativa L.) by shoot tip culture. | [Pakistan Journal of Botany • 2009]

Author(s):

1. Ren Wang: Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, (Mem. Sun Yat-Sen)Jiangsu province key laboratory for plant Ex-situ conservation, Nanjing, P.R. China

2. Li-Si He: Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, (Mem. Sun Yat-Sen)Jiangsu province key laboratory for plant Ex-situ conservation, Nanjing, P.R. China

3. Bing Xia: Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, (Mem. Sun Yat-Sen)Jiangsu province key laboratory for plant Ex-situ conservation, Nanjing, P.R. China

4. Jin-Feng Tong: Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, (Mem. Sun Yat-Sen)Jiangsu province key laboratory for plant Ex-situ conservation, Nanjing, P.R. China

5. Feng Peng: Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, (Mem. Sun Yat-Sen)Jiangsu province key laboratory for plant Ex-situ conservation, Nanjing, P.R. China

6. Ning Li: Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong SAR, P.R. China

Abstract:

This study describes the standardization of an efficient in vitro propagation and hardening procedure for obtaining plantlets from shoot tips of Hemp (Cannabis sativa L.). Hemp seedlings were germinated on half-strength 1/2 MS medium supplemented with 10 g·L-1 sucrose, 5.5 g·L-1 agar at a pH of 6.8 under light for 16 h per day. MS medium containing 0.2 mg·L-1 TDZ, 0.1 mg·L-1 NAA supported the maximal auxiliary bud multiplication rate of 3.22 per shoot tip. The proliferated buds were successfully rooted on MS medium supplemented with 0.1 mg·L-1 IBA and 0.05 mg·L-1 NAA resulting in 85% of the plantlets rooting. The procedure requires a 54 days cycle for the In vitro clonal propagation (14 days for shoot multiplication and 40 days for root induction) which includes 35-42 days for acclimatized plantlet production.

Page(s): 603-608

DOI: DOI not available

Published: Journal: Pakistan Journal of Botany, Volume: 41, Issue: 2, Year: 2009

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