Adaptation of WM-68 Hybridoma cell-line in minimal serum and serum free culture conditions. | [Pakistan Journal of Zoology • 2014]

Author(s):

1. Furqan Khurshid Hashmi: University College of Pharmacy, University of the Punjab, Lahore, Pakistan

2. Russell Cail: University of New South Wales, Sydney, Australia

3. Muhammad Islam: University College of Pharmacy, University of the Punjab, Lahore, Pakistan

4. Zikria Saleem: University College of Pharmacy, University of the Punjab, Lahore, Pakistan

5. Umair Amin: University College of Pharmacy, University of the Punjab, Lahore, Pakistan

6. Khalid Hussain: University College of Pharmacy, University of the Punjab, Lahore, Pakistan

7. Hamid Saeed: University College of Pharmacy, University of the Punjab, Lahore, Pakistan

Abstract:

WM-68 cell line has been shown to produce anti-CD48 antibody - highly specific to B cell malignancies. For their clinical usage, cultivation of WM68 cells in animal serum pose a technical and safety disadvantage. Therefore, the present study was aimed to establish an adaptation protocol for WM68 hybridoma cell line - from high to a low serum concentration and finally into a serum free culture condition. The WM68 hybridoma cell line cultured in 10% FBS exhibited excellent cell culture profiles – cell viability, density and antibody production. Similar trend was observed when WM68 cell line was cultured in 0.5% FBS. However, in 0.5% FBS, data revealed poor antibody production in the stationary phase compared to 10% FBS. Finally, WM68 cell line cultured in 0.5% FBS, was passaged and plated in a serum free condition. Unfortunately, cells didn’t survive past day one and manifested obvious signs of apoptosis. Taken together, these data suggested that 10% FBS and 0.5% FBS are suitable culture conditions for the propagation of WM68 hybridoma cell line and to generate antibodies. Thus, employing additional media factors and avant-garde cell culture techniques can enhance our understanding of the WM68 cell line culture requirements in a serum free media.

Page(s): 355-362

DOI: DOI not available

Published: Journal: Pakistan Journal of Zoology, Volume: 46, Issue: 2, Year: 2014

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