TRANSCRIPT ABUNDANCE OF HEAT SHOCK PROTEIN GENES CONFER HEAT TOLERANCE IN COTTON (GOSSYPIUM HIRSUTUM L.) | [Pakistan Journal of Botany • 2022]

Author(s):

1. MUHAMMAD MAJID ALI: Institute of Plant Breeding and Biotechnology, MNS-University of Agriculture Multan,Pakistan

2. ZULFIQAR ALI: Institute of Plant Breeding and Biotechnology, MNS-University of Agriculture Multan,Pakistan

3. FURQAN AHMAD: Institute of Plant Breeding and Biotechnology, MNS-University of Agriculture Multan,Pakistan

4. FAHIM NAWAZ: Department of Agronomy, MNS-University of Agriculture Multan,Pakistan

5. QAMAR SHAKIL: Ayub Agricultural Research Institute Multan,Pakistan

6. SAGHIR AHMAD: Ayub Agricultural Research Institute Multan,Pakistan

7. ASIF ALI KHAN: Institute of Plant Breeding and Biotechnology, MNS-University of Agriculture Multan,Pakistan

Abstract:

The one dark view of industrialization is the emission of greenhouse gases from the burning of fossil fuels which lead towards temperature elevation and global warming. Global warming is a destructive force for agriculture espacially for crop husbandry. Cotton (Gossypium hirsutum L.) is the most important natural fibre crop grown across the globe and highly vulnerable due to global warming and other climatic changes. It faces biotic and abiotic stresses throughout the world including Pakistan but heat is particularly one of the major abiotic stress that impacting cotton production negatively in Pakistan. Developing heat tolerance in cotton is one of the feasible approaches to overcome the heat problem that needs existence of variability as a pre-requisite. In the present studies, 30 cotton genotypes were assessed for their response to heat stress in randomized complete block design in two replicates at the research area of MNS-University of Agriculture Multan. Relative cell membrane injury was measured to assess heat tolerance. Total RNA was extracted from the one heat tolerant and one heat sensitive genotype. Transcript abundance analysis of HSPs genes (GhHS26 and GhHS97) was performed by RT-PCR to understand the genetic basis of heat tolerance. The CIM-616 was found to be heat tolerant while the SLH-337 heat sensitive genotype.

Page(s): 65-71

DOI: 10.30848/PJB2022-1(21)

Published: Journal: Pakistan Journal of Botany, Volume: 54, Issue: 1, Year: 2022

Keywords:

heat stress , Relative cell membrane injury , cDNA synthesis , Differential gene expression , Quantitative PCR , RNA extraction

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