A rapid Protocol for Extraction of Podophyllotoxin (An Anticancerous Compound) from Podophyllum hexandrum Royle. | [Biologia • 2017]

Author(s):

1. AMINA TARIQ: Department of Botany, Lahore College for Women University (LCWU), Lahore, Pakistan

2. SUMERA JAVAD: Department of Botany, Lahore College for Women University (LCWU), Lahore, Pakistan

3. SAFFIA BATOOL: Department of Botany, Lahore College for Women University (LCWU), Lahore, Pakistan

4. AYSHA JAVEED: Department of Botany, Lahore College for Women University (LCWU), Lahore, Pakistan

5. Khajista Jabeen: Department of Botany, Lahore College for Women University (LCWU), Lahore, Pakistan

Abstract:

Microwave assisted extraction is a modern and environment friendly approach for rapid and efficient extraction of secondarymetabolites of primaryimportance from plants. Podophyllum hexandrum is an important medicinal plant. Aryltetralin compound is obtained from this plant which is used as a precursor of a number of anticancer drugs on commercial scale. In present investigation one of the conventional extraction methods (cold maceration) and one of the modern method (microwave assisted extraction [MAE]) were compared for extraction of podophyllotoxin. Solvents used for this purpose were ethanol, ethyl acetate and n-hexane. MAE gave maximum amount of 190 mg and cold maceration gave 35.53 mg per gram of plant material with ethanol. Both the extraction methods were evaluated by quantitative analysis of podophyllotoxin using HPLC. The quantity was estimated to be higher with MAE (76 %) at 400W for 180 seconds as compared to cold maceration (67%) after 10 hours. So, Microwave assisted extraction proved to be a better analytical methods as it takes lesser time and release less energy and hazardous chemicals. It can be recommended for commercial production of podophyllotoxin as it is environment friendly.

Page(s): 73-78

DOI: DOI not available

Published: Journal: Biologia, Volume: 63, Issue: 1, Year: 2017

Keywords:

HPLC , Soxhlet extraction , Podophylotoxin , Podophyllum hexandrum

References:

[1] Ahmed , M.K.K.,,2008,,Orient Longman Private Ltd., Chennai,345,,

[2] Canela , C.,Moraesb , R.M.,Dayana , F.E,Ferreirab , D.,,2000,,,54,115,120

[3] Christenhusz , M.J.M.,Byng , J.W.,,2016,The number of known plants species in the world and its annual increase,Phytotaxa (Magnolia Press),261,201,217

[4] Delazar , A.,Nahar , L.,Hamedeyazdan , S.,Sarker , S.D.,,2012,Microwave-assisted extraction in natural products isolation,Meth. Mol. Biol,864,89,115

[5] Dobias , P.,Pavlikhova , P.,Adam , M.,Eisner , A.,Benova , B.,Ventura , K.,,2010,Comparison of pressurized fluid and Ultrasonic extraction method for analysis of plant antioxidants and their antioxidant capacity,Eur. J. of Chem,8,87,95

[6] Eskilsson , C.S.,BjˆrklundE.,,2000,Analytical scale Microwave assisted extraction,J. Chrom. A.,902,227,250

[7] Evans , W.C.,EvansPharmacognosy,,2002,,Bailliere Tindall,13,654,656

[8] Hameed , I.,Asad , U.,Murad , W.,Khan , S.,,2014,,Center of Plant Biodiversity,4,331,338

[9] Hoareau L. & DaSilva E.J.,,1999,Medicinal Plants: an emerging health aid,Elec. J. Biotechnol.,2,273,285

[10] Kaufmann , B.,Christen , P.,,2002,Recent extraction techniques for natural products: Microwave-assisted extraction and pressurized solvent extraction,Phytochem. Anal.,13,105,113

[11] Kaufmann , B.,Christen , P.,Veuthey , J.L.,,2001,Study of factors influencing pressurized solvent extraction of polar steroids from plant material,Chromatographia,54,394,398

[12] Liazid , A.,Palma , M.,Barroso , C. G.,,2007,Investigation on phenolic compounds stability during microwave assisted extraction,J. of Chrom. A.,1140,29,34

[13] Mukherjee , P.K.,,2002,,,,379,447

[14] Panday , A.,Tripathi , S.,,2014,Concept of standardization, extraction and pre phytochemical screening strategies for herbal drugs,J. of Phytochem,2,115,119

[15] Rizzi P.A.,,2004,Prentice-Hall of India Private Ltd,,,1,2

[16] Sharma , V.,,2013,Based HPLC-PDA Quantification of Podophyllotoxin in Populations of Podophyllum hexandrum Royle “Indian Mayapple” from Higher Altitude Himalayas,J. of Med,1,176,183

[17] Sultan , P.,Shawl , A.S.,Abdellah , A.A.,RamtekeP.W.,,2010,Characterization and Comparative Study on Podophyllotoxin and Related Glycosides of Podophyllum heaxandrum,J. of Biol. Sci.,2,345,351

[18] Williams , O.J.,VijayaR.G.S.,OrsatV.,Dai , J.,,2006,,,28,113,120

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