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A study of the effects of histamine, folic acid, acriflavine and indoleacetic acid on the mitotic activity of embryonic chick heart fibroblasts grown in vitro.
Author(s):
1. Wahid Hasan: Quinnipiac College, Hamden, Connecticut, USA
Abstract:
In order to study the effects of chemicals on mitosis, four chemicals, acriflavine, folic acid, histamine and indoleacetic acid were selected. The effects of these chemicals on mitotic activity were determined by observation, by counting the phases and using the formula devised by Wilson and Leduc. number of mitotically active cells Mitotic activity area of section x nuclear density of section. The results indicated that the highest mitotic activity was in the explants which contained 50,k g/ml of indoleacetic acid. All concentrations of acriflavine were strong mitotic inhibitors. Folic acid and histamine were mitotic stimulants at 50 g/ ml concentration. But 10,4g/m1 and 100,4g/m1 concentration had no effect at all. The use of indoleacetic acid, a plant growth hormone, augmented the growth of chick heart fibroblasts. The similarity of effects of indoleacetic acid on plant and animal cells, may be due to the similar mechanism of nuclear division.
Page(s): 189-196
DOI: DOI not available
Published: Journal: Pakistan Journal of Scientific and Industrial Research, Volume: 10, Issue: 3, Year: 1967
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