Abstract:
Membrane filtration (MF) is a pressure-driven separation process that employs a membrane for both mechanical and chemical sieving of particles and macromolecules. Membrane filtration, in conjunction with the U.S. EPA (Environmental Protection Business Enterprise), is a first-rate method for evaluating water since it enables the examination of a wide range of water samples in a significantly shorter amount of time. medicines, cosmetics, food, beverage, and other sectors. Membrane filters are used to sterilize heat-labile liquids and detect the presence of germs. Additionally, membrane filters play a vital role in the analysis of fine water utilizing direct microbiological counts in water engineering. With the MF method, it is simple to run 100 ml of the sample through the filtered membrane with a pore size of 47 mm. The organisms allowed to live on the membrane filter are restricted. Transfer the small amount of microbial mass into a Petri dish containing the nutritional medium through the membrane filter's bottom. Vitamins from the media placed in the Petri dish can flow through the membrane filter and reach the organisms that have been caught. Nutrient flow promotes microbial expansion and has an impact on the development of distinct colonies. In order to validate the kind of the organism present in the water, the culture plate can also be examined using confirmatory or selective media. Escherichia coli was counted using a membrane filter process that was developed and tested. The procedure quantifies E. coli within 24 hours without the need for cultures or isolate identification. It contains a primary selective differential medium for lactose-fermenting, gram negative bacteria. As a result, the membrane filtering method is a useful technique for isolating and counting microorganisms from fluid that has been polluted by microbes. The advantage of the membrane filtration method over the MPN method is that it counts a wide range of distinct colonies to estimate the microbial population. The MPN methodology, in contrast to the membrane filtration method, bases its determination of the microbial density solely on the turbidity of the conventional broth.
Page(s):
10-10
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023
Keywords:
Water purification
,
Water purification technique
,
Water Engineering
,
MPN
,
Culture isolation