The synergistic effect of miR-203 and cytarabine on the inhibition of cell proliferation and induction of apoptosis in chronic myelogenous leukemia cells | [Pakistan Journal of Pharmaceutical Sciences • 2024]

Author(s):

1. Shanshan Qi: Department of Oncology, Shijiazhuang Traditional Chinese Medicine Hospital,ShiJiaZhuang, HeBei,China

2. Jianghua Huang: Department of Pathology, Liuzhou Worker's Hospital,Liuzhou, Guangxi Zhuang Autonomous Region,China

3. Run Long: Digestive Endoscopy Department, Hebei Province Hospital of Traditional Chinese Medicine, Hebei Key Laboratory of Turbidity Toxin Syndrome,ShiJiaZhuang, HeBei,China

Abstract:

Cytarabine (Ara-C) is a commonly used chemotherapeutic drug for the treatment of leukemia, known for its significant tolerability. The down regulation of miR-203 in leukemia cells suggests its potential involvement in the pathogenesis of leukemia. In this study, we investigated the effects and possible mechanisms of miR-203 and Ara-C on proliferation and apoptosis of human leukemia K562 cells which were cultured with Ara-C and/or with transfection of miR-203 expression vectors. Our results showed that the combination of Ara-C and miR-203 synergistically inhibited the proliferation of K562 cells and the sensitivity of leukemia cells to Ara-C was increased by 2.5-fold with trasfection of miR-203. The proportion of apoptotic cells in the Ara-C and miR-203 combination group was higher than Ara-C or control plasmid group. Caspase-3 and caspase-9 activities were increased in Ara-C and miR-203 combination group. miR-203 down regulated the protein level of Bcr/abl in K562 cells compared with plasmid control. In conclusion, Ara-C in combination with miR-203 has a synergistic effect of proliferation inhibition and apoptosis induction in chronic myelogenous leukemia K562 cells, which may be associated with miR-203 down regulating Bcr/abl, thereby inhibiting cell proliferation and promoting cell apoptosis.

Page(s): 1019-1025

DOI: 10.36721/PJPS.2024.37.5.REG.1019-1025.1

Published: Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 37, Issue: 5, Year: 2024

Keywords:

Apoptosis , Leukemia , Bcrabl , cytarabine , miR203

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