Abstract:
Multidrug resistance (MDR) is a phenomenon in which the infectious agents develop resistance against more than one class of antimicrobial drugs. MDR is a serious health issue all over the world. Antibiotics resistance has reached to an alarming level with the emergence of resistance against every new drug, which is globally affecting the lives of millions of people. Urinary tract infections (UTI) are common infectious diseases affecting150 million people all over the world each year. During this research project, antimicrobial activity of different synthetic compound/ nanoparticles will be determined against UTI causing bacteria. These nanoparticles of Zn and Fe will be synthesized by green synthesis. For preliminary screening, UTI bacterial strains will be isolated from clinical samples then identified and characterized by biochemical testing. Micro-dilution method will employed to measure the minimum inhibitory concentrations (MICs) of the active constituents by MTT assay. Various mechanistic and morphological studies will also be conducted with selected potent reproducible inhibitors of MDR to evaluate their therapeutic potential. These studies will be included such as membrane potential study by flow cytometery, fluorescent microscopy, scanning electron microscopy (SEM), atomic force microscopy (AFM), study of generation of reactive oxygen species (ROS), hemolytic activity and ex-vivo analysis of respective synthetic compounds nanoparticles. They will be also be helpful inkey understanding about the mechanism of action of the newly identified antimicrobial agents and selective nanoparticles. In atomic force microscopy (AFM), scanning electron microscopy (SEM), and florescent microscopy (FM), changes in the cell surface, cell topology and viability of cells will be observed after the treatment of bacterial cells with the potent antibacterial inhibitors. ROS will also play a significant role in the destruction of cellular morphology, and finally lead to cell death. The synthetic compounds or nanoparticles will also be examined through ex vivo studies and hemolytic assay in order to evaluate their activity in the presence of human blood. Finally, the Synergistic effect of nanoparticles and Synthetic compounds in combination of commercially available drugs and Fraction inhibitory concentration index (FICI) will be evaluated. This extensive study will be conducted first time in the region of KPK, Pakistan. Following are the road map of complete research proposal. The bacterial strain of E. coli ATCC 25922 and E. coli ATCC 35218 will be obtained from the American Type Culture Collection, and used as the standard strains to evaluate the antibacterial activity of chemical compounds as well as nanoparticles. 100 duly characterized multidrug resistant clinical isolates will be obtained from the different hospitals and clinical labs of Khyber PakhtunKhaw, Pakistan. These clinical isolates will also be checked against different classes of antibiotics, such as amikacin, sulphamethoxazole, trimethoprim, ofloxacin, fosfomycin, ceftriaxone, polymyxin B, nitrofurantoin, cefizox, cefuroxime, vancomycin, levofloxacin, nalidixic acid, and gentamicin. Future Aspects: This study will be contributed in the identification of new compounds/ nanoparticles active against MDR UTI bacterial strains. Further research is will be needed to evaluate the therapeutic potential of newly identified MDR inhibitors as lead molecule. It is anticipated that this valuable research will be helpful in the area of drug discovery and development against MDR UTI infection causing bacteria.
Page(s):
173-173
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023