Abstract:
Marburg Virus Disease (MVD) is a life-threatening hemorrhagic fever, caused by two viruses-Marburg virus (MARV) and Ravn virus (RAVV)-that belong to the family Filoviridae. MVD represents a significant threat to public health owing its high mortality rate and the lack of approved vaccines. In this study, immune informatics tools were used to identify antigenic and conserved regions in viral glycoproteins, enabling the design of a chimeric multi-epitope subunit vaccine to reduce MVD-related mortality. Epitopes for cytotoxic Tlymphocytes, helper T lymphocytes, and B lymphocytes were predicted, and three highly conserved, immunogenic, and antigenic epitopes for each type were selected from both viruses. The selected epitopes were assembled into a chimeric multi-epitope vaccine construct using suitable adjuvants and linkers to enhance its immunogenicity. The designed vaccine construct demonstrated high antigenicity, non-allergen city, solubility, and favorable physicochemical properties. Computational modeling of the vaccine’s three-dimensional structure and molecular docking analysis with Toll-Like Receptor-4 indicated a strong interaction, suggesting robust immune activation potential. Furthermore, codon optimization and in silico cloning confirmed the efficient expression of the vaccine construct in the Escherichia coli K-12 strain. Immune simulations prognosticated the induction of primary and secondary arms of immune responses, such as interleukins, cytokines, and antibodies, predicting the ability of the vaccine to induce protective immunity. Although these encouraging in silico results form a solid basis, additional in vitro and in vivo experiments are indispensable to complete the validation of the safety, immunogenicity, and protective efficacy of this vaccine candidate, opening the door to future achievements in vaccine research.
Page(s):
150-150
DOI:
DOI not available
Published:
Journal: 1st International Conference on "Recent Advances in Green Biotechnology and Climate Resilience", September 15-16, 2025, Volume: 1, Issue: 1, Year: 2025
Keywords:
Vaccine
,
Epitopes
,
Marburg Virus Disease MVD
,
TollLike Receptor4