Author(s):
1. Tayyiba Afzal:
Department of Botany, PMAS Arid Agriculture University Rawalpindi, Pakistan
2. Yamin Bibi:
Department of Botany, PMAS Arid Agriculture University Rawalpindi, Pakistan
3. Syeda Sobia Gilani:
Department of Botany, PMAS Arid Agriculture University Rawalpindi, Pakistan
4. Aqsa Jabeen:
Department of Botany, University of Agriculture,Faisalabad,Pakistan
Abstract:
Medicinal plant species have significant contribution in pharmaceutical industries being producers of compounds utilized as precursors for drug development. The use of plant species in treatment of burns and infectious diseases is common in traditional medicine. The development of new antimicrobial agents against resistant pathogens is increasing interest. A plant species of Lamiaceae family; P. foetida had not been investigated for its anti-microbial potential. The preliminary phytochemical analysis of crude methanolic extracts and fractions of P. foetida leaves revealed that plant is rich in phenolic and flavonoid classes of secondary metabolites and tannins were detected in crude methanolic extract. Anti-bacterial and anti-fungal activity was tested using agar dilution technique and disc diffusion method respectively. The antibacterial potential of 5, 10 and 15 µg/ml Serial dilutions of plant extract and fractions of P. foetida was tested at four bacterial strains; Escherichia coli, Staphylococcus aureus, S. epidermis and Klebsiella pneumonia. Streptomycin sulphate (10 µg ml) used as positive control and methanol solvent (100 µg mlG) used as negative control. Similarly anti-fungal activity was tested against Aspergillius flavus and Alternaria alternate at same concentrations and plant samples. DMSO was taken as negative control while and fluconazole served as positive control. The maximum ZOI was of 34 and 33 mm was observed by crude extract at 15 µg/ ml against S. aureus and E. coli respectively. Dose-dependent inhibition of mycelial growth was observed by all samples. While Crude extract showed 16 mm inhibition at 15 µg/ ml against A. flavus and 14 mm by n-hexane F. It is expected that significant anti-microbial activity of P. feotida can lead to the advanced research towards potent natural antibiotic discovery. Therefore, the isolation and characterization of antibacterial components will be highly advantageous.
Page(s):
0-0
DOI:
DOI not available
Published:
Journal: First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book), Volume: 0, Issue: 0, Year: 2022
Keywords:
antibacterial
,
secondary metabolites
,
Antifungal
,
Fractions
,
Pseudocaryopteris foetida
References:
References are not available for this document.
Citations
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