Abstract:
Melatonin is a pleotropic molecule, regulating key biological pathways in plant including growth, rooting, seed germination, photosynthesis and defense against biotic and abiotic stresses. Moringa oleifera, commonly known as drumstick trees, is a nutritional plant found across the globe. The current study aims to evaluate the effect of melatonin on call ogenesis and regeneration in Moringa and to analyze the biochemical and metabolomics profile of the calli and regenerated plants. Moringa seeds were cultured on MS basal medium containing 3% sucrose, 0.9% agar supplemented with different concentrations of melatonin (0, 5, 10, 15, 25, 35 and 50 mg/L) and incubated at 25 ± 1°C for germination. Next, Moringa seeds were cut down into 2 to 4 pieces and thencultured on the same medium with addition of BAP (1.0 mg/l) and 2,4-D (2.0mg/l). Further, color, texture, fresh and dry biomass of the calli were evaluated. In addition, the seedlings were explored for their morphological and biochemical attributes. The results revealed that melatonin supplemented with BAP and 2,4-D, significantly improved the quality and biomass production. Biochemical characterization shows that enhanced concentration of phenolics, alkaloids and terpenoids were produced in the in vitro Moringa plant and callus. The current study concludes that melatonin as an effective elicitor in improving the quality and quantity of bioactive compounds in M. oleifera cultures. However, further studies are needed to explore the molecular pathways, involved in melatonin-induced metabolites production, contributing to the production of plant-derived pharmaceuticals.
Page(s):
110-110
DOI:
DOI not available
Published:
Journal: 1st International Conference on "Recent Advances in Green Biotechnology and Climate Resilience", September 15-16, 2025, Volume: 1, Issue: 1, Year: 2025
Keywords:
in vitro culture
,
Moringa oleifera
,
Melatonin
,
Bioactive metabolites