A survey was conducted to collect, identify and analyse biodiversity of genus Camponotus (Formicidae: Hymenoptera) collected from various regions of Khyber Pakhtunkhwa, Pakistan. Ants were collected with the help of aspirator, hand picking and pitfall trap from weeds, shrubs, ground surface, trees, agricultural fields, orchards, decaying plants etc. throughout the year 2017-2020. Preserved ants in museum were also utilized during this study. Identification was done with the help of trinocular microscope, Nikon SMZ 745T mounted with Nikon DS F-i2 camera. From results it was confirmed that 6 species of genus Camponotus viz., C. sericeus, C. invidus, C. compressus, C. parius, C. dolendus, and C. oblongus are new additions to the knowledge of Formicidae encountered from different areas of Khyber Pakhtunkhwa, Pakistan. All the species were photographed in layers of different focal lengths, focusing specimen at variable depths. Layers of images were combined together to form a single sharp image from anterior, dorsal and lateral sides of the specimen. Key to the species of genus Camponotus from Khyber Pakhtunkhwa along with its diagnostic characters, distributional records and spatial distribution of each species in Khyber Pakhtunkhwa is provided. A checklist of earlier reported species of genus Camponotus from the Pakistan is also provided.