Use of Molecular Probes for Presumptive Diagnosis of Tuberculosis Associated with Mycobacterium Tuberculosis and Mycobacterium Bovis Infection in Antelopes in Pakistan. | [Pakistan Veterinary Journal • 2019]

Author(s):

1. Raheela Akhtar: Department of Pathology, University of Veterinary and Animal Sciences,Lahore,Pakistan

2. Maryam Sadiqa: Department of Pathology, University of Veterinary and Animal Sciences,Lahore,Pakistan

3. Muhammad Yasin Tipu: Department of Pathology, University of Veterinary and Animal Sciences,Lahore,Pakistan

4. Muhammad Rizwan Khan: Safari Zoo Lahore,

5. Asim Aslam: Department of Pathology, University of Veterinary and Animal Sciences,Lahore,Pakistan

6. Muhammad Ijaz: Department of CMS; Quality Operational Lab, University of Veterinary and Animal Sciences,Lahore,Pakistan

7. Ghulam Mustafa: Department of CMS; Quality Operational Lab, University of Veterinary and Animal Sciences,Lahore,Pakistan

8. Beenish Zahid: Department of Zoology, University of the Punjab,Lahore,Pakistan

Abstract:

The etio-prevalence of tuberculosis complex and diagnostic significance of cytokines in antelopes of Lahore, Pakistan was determined by multiplex polymerase chain reaction and cytokine ELISA. One hundred blood samples of five different types of captive zoo antelopes including Mouflon sheep, black buck, gorial, hog deer and urial were tested. The percent prevalence of Mycobacterium bovis and M. tuberculosis was 30% and 20% respectively. All five categories of antelopes were infected with M. bovis and M. tuberculosis and none of the animals was positive for M. avium. Mouflon sheep, black buck and hog deer were significantly more affected by M. bovis as compared to M.tuberculosis. While Gorial had non-significantly more M.tuberculosis as compared to M. bovis. Urial had lowest tuberculosis incidence and was equally infected by both Mycobacterium species. The levels of IFN-? and TNF-a were significantly higher in TB infected animals as compared to negative controls (P<0.05). From PCR positive animals two black bucks died later and the histopathological analysis of their lungs revealed pathognomic granuloma lesions.

Page(s): 316-319

DOI: 10.29261/pakvetj/2019.067

Published: Journal: Pakistan Veterinary Journal, Volume: 39, Issue: 2, Year: 2019

Keywords:

Antelope Cytokine profile ELISA Multiplex PCR Pakistan Wildlife tuberculosis

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