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The Efects of Various High Dosage of α- Tocopherol and Ascorbic Acid in Tris Egg Yolk Extender on Post-Thawed Sperm Quality in Tropical Brahman Bulls
Author(s):
1. Langgeng Priyanto: Department of Animal Science, Faculty of Agriculture, Sriwijaya University, South Sumatra, 30862, Indonesia; Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center, Jalan Raya Jakarta-Bogor, Bogor, 16915, Indonesia
2. Herdis: Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center,Jalan Raya Jakarta-Bogor, Bogor, 16915,Indonesia
3. Santoso: Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center,Jalan Raya Jakarta-Bogor, Bogor, 16915,Indonesia
4. Pradita Iustitia Sitaresmi: Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center,Jalan Raya Jakarta-Bogor, Bogor, 16915,Indonesia
5. Tri Puji Priyatno: Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center,Jalan Raya Jakarta-Bogor, Bogor, 16915,Indonesia
6. Rahma Isartina Anwar: Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center,Jalan Raya Jakarta-Bogor, Bogor, 16915,Indonesia
7. Desiana Ade Mahari: Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center,Jalan Raya Jakarta-Bogor, Bogor, 16915,Indonesia
8. Florentina Bety Indah Lupitasari: Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center,Jalan Raya Jakarta-Bogor, Bogor, 16915,Indonesia
9. Maman Surachman: Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center,Jalan Raya Jakarta-Bogor, Bogor, 16915,Indonesia
10. I Wayan Angga Darmawan: Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center,Jalan Raya Jakarta-Bogor, Bogor, 16915,Indonesia
11. Arfan Abrar: Department of Animal Science, Faculty of Agriculture, Sriwijaya University, South Sumatra, 30862, Indonesia
12. Arum Setiawan: Department of Biology, Faculty of Mathematics and Natural Sciences, Sriwijaya University, South Sumatra, 30862, Indonesia
Abstract:
The primary causes of sperm quality decline during the freeze-thaw pathway are the peroxidation hazard caused by reactive oxygen species produced by the biological molecules of sperm. Alpha-tocopherol (Vitamin E) and ascorbic acid (Vitamin C) are two potent antioxidants that operate to prevent oxidation processes. The objective of this study was to analyze the effects of a- tocopherol and ascorbic acid on the motility, viability, abnormality, and plasma membrane quality of post-thawed Tropical Brahman bull sperm. Sperm samples were obtained and pooled from eight sexually mature Tropical Brahman Bull, separated into seven equal proportions, and diluted with TEY extender Semen was supplemented with 0 (C0;E0), 5 (C5;E5), 10 (C10;E10), and 15 (C15;E15) mg/mL of each a-tocopherol and ascorbic acid, respectively. Total sperm motility, viability, abnormalities, and semen membrane plasma were analyzed after thawing (%). C5 and E15 extenders resulted in higher total motility (p 0.05) than the other extenders, with the exception of C10 and C15, which were lower than that of the control. The extender E15 (P <0.05) exhibited the highest motility. Furthermore, compared with the other extenders, the C5 and E15 extenders led to better vitality and membrane plasma in post-thawed spermatozoa (p 0.05). Finally, compared with the other extenders, abnormalities were lower in the C5-and E10 extenders (p<0.05). Finally, the current findings show that C5 and E15 can increase the quality of post-thawed Brahman bull spermatozoa.
Page(s): 2097-2105
Published: Journal: Pakistan Journal of Zoology, Volume: 56, Issue: 5, Year: 2024
Keywords:
Morphology , Ascorbic Acid , Sperm , Tocopherol , Tropical bull
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