Hepatoprotective Evaluation of Glycyrrhiza glabra L. Root Essential Oil against Hepatotoxcity Induced by Isoniazid and Rifampicin in Mice | [Abstract Book on International Conference on Sustainable Agriculture and Food Security, August 27-31, 2023 • 2023]

Author(s):

1. Arfa Basharat: Department of Pharmacology and Toxicology, University of Veterinary and Animal Sciences Lahore, 54000,Punjab, Pakistan

2. Muhammad Adil Rasheed: Department of Pharmacology and Toxicology, University of Veterinary and Animal Sciences Lahore, 54000, Punjab, Pakistan

3. Muhammad Ovais Omer: Department of Pharmacology and Toxicology, University of Veterinary and Animal Sciences Lahore, 54000, Punjab, Pakistan

4. Muhammad Yasir Zahoor: Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences Lahore, 54000, Punjab, Pakistan

5. Qamar Niaz: Department of Pharmacology and Toxicology, University of Veterinary and Animal Sciences Lahore,54000, Punjab, Pakistan

Abstract:

Isoniazid (INH) and rifampicin (RFM) are the drugs used in the treatment of tuberculosis. These drugs cause hepatotoxicity in patients. The present study was carried out to evaluate hepatoprotective evaluation of Glycyrrhiza glabra L. root essential oil against experimentally induced hepatotoxicity with INH and RFM in mice. The root essential oil was extracted through hydro-distillation. Phytochemical constituents were analyzed through Gas chromatography-Mass spectrometry (GC-MS). Total phenolic contents were determined. Antioxidental potential of root essential oil was determined through DPPH free radical scavenging and Ferric Reducing Antioxidant Power Assay (FRAP) Assay. For invivo hepatoprotective evaluation thirty six mice were allocated into six groups having 6 mice in each group. Group A received normal saline, group B received INH and RFM (200 mg/Kg/day of each), group C received INH, RFM and Silymarin (200 mg/Kg/day of each). Groups D, E, F received Glycyrrhiza glabra root essential oil (10, 20 and 30 mg/Kg/day) along with INH and RFM (200 mg/Kg/day of each). All the treatments were through oral for 30 days. Total phenolic content was reported as 7.14mg GAE/g. DPPH assay with five concentration (50, 100, 200, 400 and 600µg/ml) were prepared and ascorbic acid was run as a standard showed highest percentage 72.21 % at 600ug/ml. Ascorbic acid IC50 was 34.0µg/ml and Glycyrrhiza glabra root essential oil IC50 was 37.32µg/ml. All the concentrations of Glycyrrhiza glabra root essential oil reduced elevated serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and bilirubin levels (P<0.05) when compared with non-treated groups. Histopathological examination revealed reduction in severity of liver damage. It is concluded that Glycyrrhiza glabra L. root essential oil might be considered as adjuvant drug in treatment of liver disorder and or as hepatoprotective therapy with anti-tubercular drugs.

Page(s): 25-25

DOI: DOI not available

Published: Journal: Abstract Book on International Conference on Sustainable Agriculture and Food Security, August 27-31, 2023 , Volume: 0, Issue: 0, Year: 2023

Keywords:

isoniazid , hepatoprotective , DPPH assay , rifampicin , Glycyrrhiza glabra L , FRAP assay , root essential oil

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