Production of polyhydroxyalkanoates (PHA) by Bacillus and Pseudomonas on cheaper carbon substrates | [Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 • 2023]

Author(s):

1. Noreen Iftikhar: Department of Life Sciences, University of Management and Technology,C-II Johar Town, Lahore,Pakistan

2. Faiza Quddus: Department of Life Sciences, University of Management and Technology,C-II Johar Town, Lahore,Pakistan

3. Navera Nadeem: Department of Life Sciences, University of Management and Technology,C-II Johar Town, Lahore,Pakistan

4. Muhammad Usama Raza: Department of Life Sciences, University of Management and Technology,C-II Johar Town, Lahore,Pakistan

5. Muhammad Zaid: Department of Life Sciences, University of Management and Technology,C-II Johar Town, Lahore,Pakistan

Abstract:

Polyhydroxyalkanoates are biodegradable, with similar physicochemical properties to synthetic plastics, and meet the Sustainable Development Goals. Two bacterial strains: Pseudomonas aeruginosa and Bacillus subtilis were selected to produce the PHA and were confirmed as PHA producers using Nile blue A and Sudan black B staining by spread plate assay as well as microscopically using Sudan stain. Banana peels and molasses were used as cheaper carbon substrates while glucose was used as the standard carbon source. The carbohydrate concentration was estimated by Phenol-sulfuric acid assay in banana peel extract (9.8mg/ml) and molasses (29.4mg/ml). In batch fermentation, time profiles of growth and PHA extraction of two strains for every 3hr were performed up to an incubation period of 48 hours at 150rpm. PHA was extracted by SDS digestion and chloroform extraction method. The PHA yield after 48hr of incubation by Pseudomonas aeruginosa was 0.380g/L (44.60%) and 1.120g/L (66.82%) and in Bacillus subtilis was 0.288g/L (39.18%) and 0.476g/L (53.84%) utilizing banana peels and molasses respectively. The chemical characterization by FTIR spectroscopy confirmed the produced monomers as Polyhydroxybutyrate (PHB) having a C=O and CH3 group at a range of 1697.8cm-1 to 1750.0cm-1 and 1395.9 cm-1 to 1458.3 cm-1 respectively. The unpaired t-test defined the significant variance between produced PHA, for Pseudomonas aeruginosa, a significant difference was observed between glucose with both substrates, while for Bacillus subtilis, a significant difference was noted only between glucose and peel extract.

Page(s): 12-12

DOI: DOI not available

Published: Journal: Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 , Volume: 0, Issue: 0, Year: 2023

Keywords:

Pseudomonas , molasses , Bacillus , Banana peels , PHA yield

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