Synthesis of selenium nanoparticles using root extract of zingiber officinale and evaluation of their antibacterial activity against escherichia coli | [Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23 • 2023]

Author(s):

1. Ayesha Qaisar: Department of Zoology,Wildlife and Fisheries

2. Syeda Maryam Hussain: Department of Livestock Production and Management.

3. Muhammad Zishan Ahmad: Department of Veterinary Pathology

4. Naveed Iqbal Raja: Department of Botany

5. Murtaz Ul Hassan: Department of Parasitology and Microbiology

6. Muhammad Shoaib: Department of Parasitology and Microbiology

7. Saif Ur Rehman: Department of Parasitology and Microbiology

8. Aayesha Riaz: Department of Parasitology and Microbiology

9. Zaib Ur Rehman: Department of Poultry Science, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan

10. Ali Ahmad: Microbiology Section, Department of Pathobiology, Narowal Campus, University of Veterinary & Animal Sciences, Lahore, Pakistan

11. Zahid Manzoor: Department of Parasitology and Microbiology.

Abstract:

Poultry industry in Pakistan is a thriving agricultural sector, meeting the demand for affordable meat and serving as a significant protein source. However, disease outbreaks and the widespread use of antibiotics have contributed to the emergence of antibacterial resistance, posing a global concern for healthcare authorities. Responsible antibiotic use, along with alternative strategies like nanoparticlesis crucial to mitigate resistance development and safeguard public health in the poultry industry. This study aimed to assess the potential of selenium nanoparticles (SeNPs), produced through phytosynthesis using the root extract of Zingiber officinale, in inhibiting the growth of pathogenic bacteria, specifically Escherichia coli (E.coli). Various characterization techniques such as Ultraviolet–visible spectroscopy (UV-Spectroscopy), X-ray diffraction (XRD), Energy Dispersive XRay Analysis (EDX), Fourier Transform Infrared Spectroscopy (FTIR), and Scanning Electron Microscopy (SEM) were employed to analyze the synthesized SeNPs. The results indicated that the nanoparticles were approximately 80-100 nm in size, exhibited a nearly spherical shape, and possessed functional groups as well as stability properties as revealed by FTIR analysis. The antibacterial efficacy of SeNPs against E.coli was evaluated using the disc diffusion method at various concentrations. The findings demonstrated that the phytosynthesizedSeNPs effectively inhibited the growth of E.coli, exhibiting the largest zone of inhibition with a diameter of 22mm and the smallest zone of inhibition with a diameter of 9.5mm. These results suggest that phytosynthesized selenium nanoparticles hold promise as an alternative to conventional antimicrobial agents for managing colibacillosis

Page(s): 106-106

DOI: DOI not available

Published: Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023

Keywords:

Escherichia coli , Zingiber officinale , Colibacillosis , Selenium nanoparticles

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