Abstract:
World population in increasing day by day and global crop production is becoming more and more necessary to meet the demands of this expanding population, and by 2050, it is predictable to reach almost 9 billion people. Worldwide agriculture is transitioning to a more sustainable and environmentally friendly strategy, spurred by rising demand and knowledge of the detrimental effects existing agricultural practices have on health of human being and the environment. Since Pakistan is an agricultural country and it contributes above 18% to Pakistan's GDP. But this increasing population and food demand also resulted in increased industrial practices. Heavy metals released from the these industries are contaminating our environment including soil and water. So there is a dire need to eradicate this problem to protect our environment as well as our agriculture. Plant growth promoting bacteria are the rhizobacteria, affecting the growth of plants. Some of the PGPR are able to resist the heavy metals, although majority of them are sensitive to the heavy metals. The current study deals with the evaluation of chromium resistant plant growth promoting rhizobacteria for growth promotional potential by applying post germination treatment technique using soil and foliar application to Zea mays L. plant. The treated and non-treated plants were then analyzed for the various growth aspects and biochemical parameters to observe the impact of chromium resistant plant growth promoting rhizobacteria on plant growth. The bacterial strains were also characterized genetically by extracting the DNA of the Cr-tolerant and Cr-sensitive samples. The extracted DNA was then analyzed by running the sample using gel electrophoresis system and gel documentation system. Result showed that among six bacterial strains, Th6, Tp8, A9G and DS4 were chromium resistant and Chromate reductase gene was present on the plasmids of these chromium resistant bacteria while chromium sensitive strains 3a and Th1 lacked it. Among chromium resistant strains, DS4 showed significant enhancement in the Zea mays L. growth.
Page(s):
140-140
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
PGPR
,
Zea mays L
,
Chromium
,
chromate reductase
,
Phytoremediatuon