Abstract:
Medicinal plants have long been studied due to their anticancer effects and use of them is commonly increased as a complementary and alternative medicine among patients with cancer. As drugs obtained from plants are cheap, safe to use, available without any difficulty, more effective, easy to store, and rarely have side effects. Alhagi maurorum, a member of family Fabaceae contained many secondary metabolites and used in folk medicines as laxative, purgative, diaphoretic, expectorant and diuretic. Extracts were prepared by cold maceration technique. Different extracts (Crude, Methanol, Ethyl acetate and n-Hexane) of A. maurorum from aerial part were screened for Total Phenolic, Tannins, Alkaloids content, Antibacterial, Antifungal and Cytotoxic activities. n-Hexane extract showed high TTC, TPC and TAC content. Cytotoxic properties were examined by Brine Shrimp Lethality Assay. Different concentrations (1000, 750, 500, 250, 100 and 50 µg/mL) of Methanol, Ethyl acetate and n-Hexane extract were tested against 10 nauplii. Cytotoxic activity was dose dependent. LD50 of following extracts was 381.40 ppm, 160.27 ppm and 385.72ppm respectively. The antimicrobial activity was determined against 4 pathogenic bacterial strains (Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas sp.) using Agar Well Diffusion Method. All extracts showed concentration dependent inhibition against tested strains. The maximum Zone of Inhibition was observed by Crude (11.3±1.1, 10.3±0.5) and ethyl acetate (10.3±0.5, 10±1.0) at 100µg/ml against Staphylococcus aureus, Staphylococcus epidermidis respectively. Antifungal action of Methanol,and nHexane,extracts (10, 25, 50µg/ml) were examined by Agar Tube Dilution method against Fusarium and Alternaria sp. At 50µg/ml highest percentage inhibition was observed by Methanol extract for both the strains i.e. 91.8% for Alternaria sp. and 80% for Fusarium sp. The present study revealed that A. maurorum carries powerful antimicrobial and remarkable cytotoxic activities, could be a potential source of antibiotics and anticancer compounds. This plant ought to be considered for additional phytochemical examination and pharmacological assessment.
Page(s):
0-0
DOI:
DOI not available
Published:
Journal: First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book), Volume: 0, Issue: 0, Year: 2022