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Antioxidant and Antibacterial Activity of Plants used for Making Herbal Teas in District Haveli, Azad Kashmir, Pakistan
Author(s):
1. Hadia Shafi: Department of Botany, University of Poonch Rawalakot,Azad Kashmir,Pakistan
2. Ansar Mehmood: Department of Botany, University of Poonch Rawalakot,Azad Kashmir,Pakistan
Abstract:
Herbal tea has been consumed for centuries due to its health advantages and delicious flavor. Herbal tea is basically an herbal mixture prepared from leaves, seeds or roots of several plants. The objective of the study was to measure the antioxidant and antimicrobial activity of Berginia ciliata, Calamus rotang, Mentha arvensis, Polygonum viviparum, and Thymus linearis, commonly used herbal plants for making herbal tea in District Haveli, Azad Kashmir. Total phenolic content (TPC) and total flavonoid content (TFC) were calculated in phytochemical analysis. Antioxidant activity was measured using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay, hydrogen peroxide (H2O2) assay, and reducing power assay. The disc diffusion method was used to determine antibacterial activity against gram-positive and gram-negative microorganisms. The phytochemical analysis showed highest TPC in extract of C. rotang (354.12±1.65 mg GAE/g) followed by B. ciliata, M. arvensis, T. linearis and P. viviparum, respectively. Highest TFC were found in M. arvensis (162.8±1.50 mg QE/g) extract obtained by decoction. The extract obtained by decoction method of P. viviparum showed highest antioxidant activity with the lowest IC 50 value (27.19 µg/ mL) against DPPH. Against H2O2, the extract obtained by maceration method of Thymus linearis showed highest antioxidant activity by the IC 50 value of 26.81 µg/mL. In reducing power assay, the extract obtained by maceration method of Polygonum viviparum showed highest activity with the IC 50 value of (62.70 µg/mL) as compared to decoction process. The maximum antibacterial activity (16.67±0.88 mm) was shown by extract of M. arvensis obtained through maceration process against Pseudomonas aeruginosa as compared to Staphylococcus aureus. The study established that the biological activity of herbal teas might be used for curing of various infectious diseases.
Page(s): 0-0
DOI: DOI not available
Published: Journal: First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book), Volume: 0, Issue: 0, Year: 2022
Keywords:
Antibacterial activity , Antioxidant activity , NULLHerbal teas
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