Abstract:
-Ascorbic acid, commonly called vitamin C, is water soluble and an important antioxidant for the body. Due to the COVID-19 pandemic, a reliable source of antioxidants has been increased. Consumers and quality control agencies now need reliable information about the levels of vitamin C in various local fruits. . This study aims to find out if Vitamin C is readily available in tropical fruits (Mango, Banana, Guava, and Papaya) and stone fruits (cherry, plum, and peach). The study was carried out by collecting these fruits in their fresh state by using the traditional titration method and modern analytical techniques, UV-Visible spectrophotometer, and FTIR. Ascorbic acid content was calculated in titration, iodine reacts with ascorbic acid, which then reacts with starch solution and the endpoint is deep blue coloration. In the UV-visible technique, bromine water reacts with ascorbic acid, and then it is then treated with 2, 4-DNPH at room temperature for three hours. The solution was treated with 95% H2SO4 and the absorbance was noted at 521nm. The same solutions prepared for the UV-Visible spectrophotometer were used in FTIR. The samples prepared were stored for one week and examined under a UV-Visible spectrophotometer and FTIR. The samples were again prepared and examined after three days. By comparing both results it was found that the Vitamin C concentration decreases during storage. The outcome of the study suggests that these methods of examination are cheap and reliable for routine analysis. However, the traditional titrimetric method is more time-consuming and less precise in comparison to analytical methods UV-Visible and FTIR which are more precise and less time-consuming.
Page(s):
0-0
DOI:
DOI not available
Published:
Journal: First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book), Volume: 0, Issue: 0, Year: 2022
Keywords:
ascorbic acid
,
Antioxidant
,
UVVisible
,
Titration